二氯喹啉酸在土壤、畸形烟叶和烤烟中残留量的比较分析

目的]为了比较分析烟叶及烤烟中二氯喹啉酸残留量,建立二氯喹啉酸在其药害土壤、畸形鲜烟叶和烤烟中的分析方法.方法]样品用30 mL 0.05 mol/L硼砂缓冲液和60 mL甲醇提取,液液分配后,采用HPLC方法检测二氯喹啉酸的残留量.结果]二氯喹啉酸在土壤中的平均回收率为98.4％～98.9％,相对标准偏差为0.7％～1.8％,在鲜烟叶中的平均回收率为87.6％～90.4％,相对标准偏差为6.7％～9.0％；在烤烟中的平均回收率为86.1％～90.2％,相对标准偏差为6.0％～7.7％.结论]该方法操作简单、准确,重复性好,利用二极管阵列检测器灵敏度较高.经方法分析二氯喹啉酸在其药害土壤、鲜烟叶及烤烟中残留量分别为0.1306、0.2575、0.0742mg/kg.

The Comparative Analysis of Residues of Quinclorac in Soil,Deformity and Flue-cured Tobacco

It was to compare the residues of quinclorac in deformity and flue-cured tobacco,and establish the analysis method of quinclorac residues in its phytotoxicity soil,deformity and flue-cured tobacco.Methods] Residues of quinclorac were extracted from samples with 0.05 mol/L borax buffer and 60 mL methanol,after liquid-liquid partition,and determined with HPLC.Results] The average recovery rate of quinclorac in soil was 98.4-98.9%,the relative standard deviation was 0.7-1.8%.The average recovery rate of quinclorac in deformity and flue-cured tobacco were 87.6-90.4% and 86.1-90.2% respectively.Their relative standard deviation were 6.7-9.0% and 6.0-7.7% respectively.Conclusions] This method is simple and accurate with good repeatability and high sensitivity using diode array detector.The residues of quinclorac on its phytotoxicity soil,deformity and flue-cured tobacco are 0.1306,0.2575,0.0742 mg/kg respectively.