内蒙古白绒山羊IGF-IR基因cDNA克隆及组织表达特异性分析

旨在克隆内蒙古白绒山羊IGF-IR基因并分析其基本表达模式.采用RT-PCR克隆基因,将得到的IGF-IR基因cDNA片段的核苷酸序列及其编码的氨基酸序列进行生物信息学分析.半定量RT-PCR进行组织特异性表达检测.获得了内蒙古白绒山羊IGF-IR基因3’端编码区2118 bp的cDNA序列(JN200823),编码705个氨基酸残基.核苷酸序列与牛的IGF-IR( XM606794.3)基因同源性为98％,相应的氨基酸序列同源性为99％.SMART分析表明,推导出的编码蛋白具有跨膜域,酪氨酸激酶催化域.半定量RT-PCR检测表明,IGF-IR基因在绒山羊脑、胰腺、肝、肾组织中均有表达.

Cloning and Tissue-specific Expression Pattern of IGF-IR Gene cDNA in Inner Mongolia Cashmere Goat

The present study aims at cloning the code sequence fragment of IGF-IR gene cDNA from Inner Mongolia cashmere goat and analyzing its tissue-specific expression pattern.IGF-IR gene cDNA fragments were cloned by RT-PCR.The nucleotide sequence was analyzed by BLAST while amino acid sequence was analyzed by online soft wares SMART.The tissue-specific expression pattern of IGF-IR gene was analyzed by semi-quantitative RT-PCR.The cloned 3’ terminal 2 118 bp IGF-IR gene cDNA(JN200823)from Inner Mongolia Cashmere goat was a part of coding region,encoding 705 amino acid residues.The nucleotide sequence shares 98% identity with Bos tuarus IGF-IR gene(XM606794.3)and the amino acid sequence shares 99% identity.Analysis with SMART suggested that the deduced encoding protein contained a transmembrance domain and a TyrKc domain.The IGF-IR gene was shown to express in brain,pancreas,liver and kidney.