乳酸链球菌素工业生产过程的稳定性研究

目的 为提高乳酸链球菌素(nisin)在工业生产中的稳定性,研究不同温度、pH值、酸对nisin稳定性的影响,优化制备工艺,提高其稳定性.方法 建立高效液相色谱快速检测Nisin效价的方法并进行可靠性验证;将nisin水溶液分别于不同pH、不同温度条件下作用一定时间,定期监测其效价;使用不同的酸制备nisin成品,定期...

Stability Study on Nisin in Industrial Production

Objective To improve the stability of nisin in industrial production by studying the effects of temperature,pH and acid on the stability of nisin.Methods A method for rapid determination of nisin titer by HPLC was established and its reliability was verified.Nisin solution was prepared and placed at different pH and temperature,and the potency was regularly monitored.Nisin products were prepared with different acids,and the potency was regularly monitored.Results The standard curve of nisin showed good linearity and reliable accuracy.Nisin had good stability under the condition of pH less than 4.0,and had the best stability at pH 2.0.The lower the temperature was,the more stable the aqueous solution was.Compared with the control group,the stability of nisin products prepared with lactic acid and phosphoric acid was significantly improved.Conclusion Nisin is relatively stable at low pH and low temperature.The preparation of nisin finished products with lactic acid and phosphoric acid can improve the stability.