β-淀粉样蛋白致大鼠Alzheimer病模型的研究

目的 :模拟人类Alzheimer病 (AD)的大鼠病理模型 ,观察其学习记忆及病理性改变。方法 :大鼠双侧海马微量注射聚合态Aβ1 40 制备AD动物模型 ,Y 型迷宫测定大鼠学习记忆能力 ,HE染色、刚果红染色和银染检测病理变化。结果 :海马内注射Aβ1 40 可引起大鼠学习记忆能力下降 ,注射区Aβ沉积 ,神经元丢失及胶质细胞增生 ,神经原纤维缠结。结论 :Aβ1 40 海马内注射能较好的模拟AD的学习记忆障碍、神经元损伤等行为学和病理学等方面的特征 ,作为AD研究较好的动物模型。

The study of β-amyloid protein induced alzheimer disease model rats

Objective: To study the changes of learning and memory abilities and pathology in alzheimer disease rats. Methods: Incubated Aβ 1-40 was microinjected into the bilateral hippocampus of each rat. Y- maze was used to evaluate learning and memory ability of rats';the sections were stained with haematoxylin-eosin(HE) , Congo Red and silver staining. Results: The capabilities of learning and memory were impaired significantly after Aβ 1-40 injection. Aβ deposition ,neuron death ,glial reaction and neurofibrillary tangles were found in or around the injection sites.Conclusion: These results suggest that the aggregated Aβ 1-40 and its deposition in the hippocampus is related to the impairment of learning and memory and the neuronal degeneration in the rats which shows similar behavior and pathological characterizations of AD. This Aβ-treated rats could be used as an animal model for AD.