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响应面法优化黑果枸杞多糖的提取工艺研究
引用本文:鲁小静,冯艳波,陈晓瑞,韩爱芝,白红进.响应面法优化黑果枸杞多糖的提取工艺研究[J].中国酿造,2013,32(6):79.
作者姓名:鲁小静  冯艳波  陈晓瑞  韩爱芝  白红进
作者单位:塔里木大学生命科学学院,新疆阿拉尔843300;新疆生产建设兵团塔里木盆地生物资源保护与利用重点实验室,新疆阿拉尔843300
摘    要:优化黑果枸杞果实多糖的提取工艺.采用单因素试验,考察了水浸提取温度、时间、料液比对多糖提取率的影响规律,依据Box-Behnken的中心组合设计,采用响应面法优化从黑果枸杞中提取粗多糖.最佳工艺条件为热水温度90℃,时间66min,料液比1∶25(g∶mL);在此条件下黑果枸杞多糖得率为29.36%.采用响应面法优化热水浸提黑果枸杞多糖的工艺条件稳定可行.

关 键 词:黑果枸杞  多糖  响应面  提取工艺  

Optimization for extraction technology of Lycium ruthenicum Murr. polysaccharides by response surface methodology
LU Xiaojing,FENG Yanbo,CHEN Xiaorui,HAN Aizhi,BAI Hongjin.Optimization for extraction technology of Lycium ruthenicum Murr. polysaccharides by response surface methodology[J].China Brewing,2013,32(6):79.
Authors:LU Xiaojing  FENG Yanbo  CHEN Xiaorui  HAN Aizhi  BAI Hongjin
Affiliation:1.College of Life Science, Tarim University, Alar 843300, China; 2.Key Laboratory of Protection & Utilization of
Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Alar 843300, China
Abstract:Optimization for the extraction technology of Lycium ruthenicum polysaccharide was focused on in this paper. The single factor experiment and response surface methodology based on Box-Behnken central composite design was used to optimize the extraction of L. ruthenicum crude polysaccharide. The optimal extraction technology were extraction temperature 100℃, extraction time 66 min, solid-to-solvent radio 1∶25 (g∶ml). Under these conditions, the extraction yield of crude polysaccharide was 29.36%. The extraction technology of L. ruthenicum polysaccharide was stable and viable.
Keywords:Lyciurn ruthenicum  polysaccharide  response surface methodology  extraction process  
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