狭叶红景天引种驯化后抗氧化能力、主要活性物质含量及挥发性组分的变化

以甘肃省甘南藏族自治州碌曲县栽培和野生狭叶红景天为材料,分别对根茎(主根和侧根)的抗氧化能力、可溶性糖、总黄酮、酚类和红景天苷含量,以及挥发性组分进行研究.结果表明: 狭叶红景天栽培根茎的自由基抑制率(I)、铁离子还原/氧化能力(FRAP),以及可溶性糖、总黄酮、酚类和红景天苷含量均显著大于野生根茎;栽培和野生根茎中均含有21种挥发性组分,相对含量较高的为苯乙酮、2-糠醛、棕榈酸等;与野生根茎相比,栽培根茎有15种组分的相对含量增加,主根中相对变化量较大的为3-羟基月桂酸、2,4-二叔丁基苯酚、亚麻酰氯等,侧根为2-乙酰呋喃、对羟苯基乙醇、丁子香酚等.栽培和野生侧根的I、FRAP值,以及总黄酮、酚类和红景天苷含量均显著大于主根.与主根相比,侧根有12种挥发性组分相对含量增加,变化量较大的为3-羟基月桂酸、10,13-十八碳二炔酸甲酯、对羟苯基乙醇等.栽培驯化后狭叶红景天根茎的抗氧化能力、主要活性物质含量及挥发性组分相对含量高于野生狭叶红景天,且侧根的品质更佳.

Changes in antioxidant capacity,main bioactive compounds and volatile constituents of Rhodiola kirilowii after introduction and acclimatization.

An experiment was conducted on the antioxidant capacity, contents of soluble sugar, flavonoids, phenolics and salidroside, and volatile constituents in rhizome (taproot and lateral root) of cultivated and wild Rhodiola kirilowii selected from Luqu County, Gannan Tibetan Autonomous, Gansu Province, China. The results showed that the inhibition rate (I) and ferric reducing/antioxidant power (FRAP) value, the contents of soluble sugar, total flavonoids, phenolics and salidroside in the rhizome of cultivated R. kirilowii were significantly higher than that of wild plant, respectively. Both of the rhizomes of cultivated and wild R. kirilowii contained 21 volatile constituents, with acetophenone, 2-furaldehyde and hexadecanoic acid occupying high relative contents. The relative changes of 15 volatile constituents in cultivated rhizome were higher compared with that of wild rhizome, such as 3-hydroxydodecanoic acid, 2,4-di-tert-butylphenol and linoleoyl choride in taproot, and 2-acetyl furan, p-hydroxyphenyl ethanol and eugenol in lateral root. I and FRAP value, the contents of total flavonoids, phenolics and salidroside in lateral root of cultivated and wild R. kiri-lowii were significantly higher than that of taproot. There were 12 volatile constituents increased in lateral root compared with taproot, such as 3-hydroxydodecanoic acid, 10,13-octadecadiynoic acid methyl ester, p-hydroxyphenylethanol. After introduction and acclimatization, the antioxidant capa-city, contents of main bioactive compounds and volatile constituents in the rhizome of R. kirilowii were superior to that of wild species, and the quality of lateral root was better than taproot.