Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts |
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| Authors: | HAO Chang Fu LI Xiao Fang YAO Wu |
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| Affiliation: | Department of Occupational Health and Occupational Disease, College of Public Health, Zhengzhou University,Zhengzhou 450001, Henan, China |
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| Abstract: | ObjectiveTo study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblastsMethodsRat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of α-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin.ResultsThe expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used.ConclusionThe IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts. |
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| Keywords: | Transdifferentiation Lung fibroblasts Insulin-like growth factor II receptor Silicosis |
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