超抗原金黄色葡萄球菌肠毒素C和肿瘤可溶性抗原诱导的细胞毒性T淋巴细胞的杀瘤效应

目的 探讨肿瘤可溶性抗原(TSA)联合超抗原金黄色葡萄球菌肠毒素C(SEC)诱导的细胞毒性T淋巴细胞(CTLs)对肿瘤细胞的杀伤作用.方法 实验分为对照组(淋巴细胞)和实验组(SEC+ TSA+淋巴细胞).分离肿瘤患者外周血淋巴细胞,经TSA、超抗原SEC联合作用诱导产生CTLs,对其增殖、细胞表型、杀瘤活性进行观察和测定.结果 经TSA、SEC联合刺激的淋巴细胞组增殖活性明显增强.实验组和对照组CD3均阳性表达,实验组CD8+明显高于对照组(49.07％比27.52％,P＜0.05).经肺癌TSA、超抗原SEC联合刺激淋巴细胞组培养诱导的CTLs,当效靶比为20∶1时对CALU-6、自体肺癌细胞、Hela细胞杀伤活性明显高于效靶比为10∶1[分别(89.6±3.7)％比(51.5±4.0)％,(92.0±4.0)％比(54.5±4.0)％,(65.0±3.8)％比(35.3±2.4)％,均P＜0.05]；效应细胞对人肺癌细胞株CALU-6、自体肺癌细胞的杀伤活性明显高于Hela细胞(P＜0.05).结论 经肿瘤抗原和超抗原SEC诱导的CTL能够产生高效特异性的杀瘤效应.

Anti-tumor effect of cytotoxic T lymphocyte cells modified by super-antigen staphylococcal enterotoxin Cand tumor soluble antigen

Objective To study the cytotoxic T lymphocyte cells（CTLs） induced by tumor soluble antigen （TSA） and super-antigen staphylococcal enterotoxin C （SEC） and its anti-tumor effect in vitro. Methods The experiment was divided into control group （ lymphocyte cells ） and experiment group （ SEC ＋ TSA ＋ lymphocyte cells）. Lymphocyte cells were isolated from peripheral blood of cancer patients and then were induced by TSA and SEC in vitro, the levels of proliferation, cell phenotype and killing activity of CTLs were tested. Results Proliferation activity of lymphocyte cells group stimulated by TSA and SEC was the strongest. CD3 was expressed in control group and experiment group, and the CDs＋ expressed in experiment group was higher than that in control group（49. 07% vs 27. 52%, P 〈 0. 05）. When the ratio of effect and target was 20： 1, the kill rates of CTLs on CALU-6, the lung cancer cells and Hela cells were higher than those when the ration was 10： 1, and the kill rates of CTLs on CALU-6, the lung cancer cells were higher than that of Hela cells （P 〈 0. 05）. Conclusion The CTLs modified by super-antigen SEC and tumor antigen can induce high anti-tumor efficiency.