低剂量棉酚与激素联合应用的抗生育作用位点的研究

目的 探讨低剂量棉酚与激素联合应用在8周内使大鼠达到不育效果的作用位点.方法 本实验采用大鼠喂服棉酚12.5mg/(kg·d) 去氧孕烯125μg/(kg·d) 炔雌醇25μg/(kg·d) 十一酸睾丸酮100mg/(kg·d)的联合用药方式,与单独喂服相同剂量的棉酚或激素的大鼠及喂服载体溶剂的大鼠相对照,通过一系列形态学观察和半定量检测,探讨联合用药的具体作用环节.结果 无论在睾丸组织形态还是睾丸精子数量,联合用药组的变化趋势都与单独应用激素组的相似,而不同于单独应用棉酚组和对照组.而在附睾中,3个用药组在精子活力和精子形态方面,都与对照组有显著差异.结论 联合用药组中激素主要使睾丸精子数量迅速下降;而在精子数量大大下降的基础上,棉酚和激素又对已成熟精子的结构和运动能力进一步破坏,从而使雄性大鼠受精能力迅速下降,联合用药组比单独应用其中一种成分更快、更有效地达到使大鼠不育的目的.

SITES OF ACTION OF COMBINATION REGIMEN OF LOW-DOSE GOSSYPOL ACETIC ACID WITH STEROID HORMONES ON MALE RAT

Objective To investigate the target sites for male rats infertility by administered with combination regimen of low dose gossypol acetic acid (GA) with steroid hormones (desogestrel/ethinylestradiol/testosterone undecanote (DSG/E/TU). Methods Adult male rats were randomly divided into four groups. Group GH: rats were fed orally with GA (125mg(kgI>&#/I>8226;day)) and DSG (0.125mg/(kgI>&#/I>8226;day))/E(0.025mg/(kgI>&#/I>8226;day))/TU (100mg/(kgI>&#/I>8226;day)); Group G: rats were administered with a single dose of GA (12.5mg/(kgI>&#/I>8226;day)); Group H: DSG(0.125mg/(kgI>&#/I>8226;day))/E(0.025mg/(kgI>&#/I>8226;day))/TU (100mg/(kgI>&#/I>8226;day)) were administered to rats; Group C: rats only received vehicle (1% methyl cellulose). The testis and epididymis were removed at 4, 6, 8, 10 weeks respectively. Morphology of testis, epididymis and sperm were observed, and testicular sperm number was counted, epididymal sperm motility was measured. Results We found that group GH had similar results as group H ,but not either as group G or as group C, in testicular sperm count and the morphology of testis seminiferous tubules, while in epididymis, the sperm motility decreased and the abnormal sperm increased obviously in all three treatment groups compared with group C.Conclusion The steroid hormones are the main functional factors on spermatogenesis in testis, gossypol and the hormones have synergetic effects on sperm motility in epididymis. With either independent or synergistic actions of gossypol and steroid hormones, the combined regimen have a promising effect on male rat contraception.