Respiration-based measurement of lung deposition of a fluorescent dextrane aerosol in a marmoset monkey |
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Authors: | Bergers Willem W A van de Meent-van der Horst Dory Joosen Marloes J A |
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Affiliation: | TNO Prins Maurits Laboratory, Division Chemical and Biological Protection, Rijswijk, The Netherlands. bergers@pml.tno.nl |
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Abstract: | A technique for measurement of respiration-based lung deposition of an aerosol was investigated and subsequently applied in a pilot study with a marmoset monkey. The technique consisted of an aerosol exposure system for a marmoset using a face mask and a previously constructed monkey chair, a method for recovery of fluorescent dextrane from lung material, and respiration measurement of the marmoset by whole-body plethysmography. In the pilot study, a ketamine-anesthetized marmoset was exposed for 20 min to an FITC-dextrane aerosol atmosphere (200 microg/L air, particle size 1.5 microm mass median aerodynamic diameter MMAD]). It was found that 3.4% of the inhaled aerosol was deposited in the lungs; the aerosol was distributed over the lung lobes with an higher concentration at the distal side. |
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