| 白介素-4基因C-33T多态性与呼吸道合胞病毒毛细支气管炎的相关性 |
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| 引用本文： | 董琳,黄志英,张亚丽,陈小芳.白介素-4基因C-33T多态性与呼吸道合胞病毒毛细支气管炎的相关性[J].临床儿科杂志,2009,27(2). |
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| 作者姓名： | 董琳 黄志英 张亚丽 陈小芳 |
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| 作者单位： | 1. 温州医学院附属育英儿童医院呼吸科,浙江温州,325027
2. 南京医科大学附属常州第二人民院儿科,江苏常州,213003 |
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| 基金项目： | 浙江省温州市科技计划项目，浙江省医药卫生科学研究基金 |
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| 摘 要： | 目的 研究白介素-4(IL-4)基因C-33T与呼吸道合胞病毒(RSV)毛细支气管炎的易感性、病情严重程度的关系及对血清总IgE和鼻咽分泌物(NPS)IL-4水平的影响.方法 采用聚合酶链-限制性片段长度多态法(PCR-RFLP)检测130例RSV毛细支气管炎患儿和108例对照组儿童IL-4/C-33T位点多态性,分别用化学发光法和酶联免疫分析法,检测RSV毛细支气管炎患儿血清总IgE和NPs中IL-4水平.结果 两组IL-4启动子区C-33T位点均可见TT、CT和CC 3种基因型,其中病例组,TT、CT和CC基因型频率分别为66.9%、26.9%和6.2%,对照组分别为69.4%、26.9%和3.7%,两组差异无统计学意义(X2=0.758,P>0.05);病例组T、C等位基因频率分别为80.3%、19.7%,对照组分别为82.9%、17.1%,两组差异亦无统计学意义(X2=0.073,P>0.05;OR=0.847,P>0.05).病例组三种基因型间NPS中IL-4及血清总IgE水平差异均无统计学意义(H=0.103,F=0.529,P均>0.05);三种基因型频率在轻度组和中重度组间的差异亦无统计学意义(X2=0.825,P>0.05).结论 温州地区儿童存在IL-4/C-33T位点的多态性,但未发现其与RSV毛细支气管炎存在关联.
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| 关 键 词： | 呼吸道合胞病毒 毛细支气管炎 基因多态性 白介素-4 |
Relationship between IL-4/C-33T gene polymorphism and respiratory syncytial virus bronchiolitis |
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| DONG Lin,HUANG Zhi-ying,ZHANG Ya-li,CHEN Xiao-fang.Relationship between IL-4/C-33T gene polymorphism and respiratory syncytial virus bronchiolitis[J].The Journal of Clinical Pediatrics,2009,27(2). |
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| Authors: | DONG Lin HUANG Zhi-ying ZHANG Ya-li CHEN Xiao-fang |
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| Affiliation: | Department of Respiratory Diseases;Yuying Children's Hospital Affiliated to Wenzhou Medical College;Wenzhou 325027;Zhejiang;China;2.Changzhou No.2 People's Hospital Affiliated to Nanjing Medical University;Changzhou 213003;Jiangsu;China |
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| Abstract: | Objectives To study the correlation between the single nucleotide polymorphism(SNPs) of interleukin-4(IL-4)/C-33T with the susceptibility to respiratory syncytial virus(RSV) bronchiolitis and the severity of RSV bronchiolitis in Chinese children.To explore the impact of such polymorphism upon the IL-4 production in nasopharyngeal secretions(NPS) and total serum IgE level.Methods Polymorphism in IL-4 gene/C-33T was analyzed by means of polymerase chain reaction-restriction fragment length polymorphism analys... |
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| Keywords: | respiratory syncytial virus bronchiolitis gene polymorphism interleukin-4 |
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