| 灯盏生脉胶囊对博莱霉素诱导的小鼠肺纤维化的干预作用 |
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| 引用本文： | 曾嫱,谭亚夏,孙红,肖丹,刘蓉,朱琦,杨通,钟南山.灯盏生脉胶囊对博莱霉素诱导的小鼠肺纤维化的干预作用[J].中国实验方剂学杂志,2013,19(11):199-203. |
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| 作者姓名： | 曾嫱 谭亚夏 孙红 肖丹 刘蓉 朱琦 杨通 钟南山 |
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| 作者单位： | 广州医学院第一附属医院呼吸疾病国家重点实验室,广州,510120 |
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| 基金项目： | 广州呼吸疾病国家重点实验室开放课题项目(2007DA780154F0908) |
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| 摘 要： | 目的:研究灯盏生脉胶囊对博莱霉素诱导的小鼠肺纤维化的干预作用.方法:将40只C57BL/6雌性小鼠随机分为盐水对照组(NS)、模型组(Model)、灯盏生脉组(DZSM)和N-乙酰半胱氨酸阳性对照组(N-AC),NS组气管滴注0.1 mL生理盐水,其余3组按5 mg·kg-1剂量滴注博莱霉素溶液复制肺纤维化模型,以后每天分别给予生理盐水、灯盏生脉溶液(222mg· kg-1·d-1)、N-乙酰半胱氨酸溶液(489.6 mg·kg-1)ig治疗,连续给药21 d.实验过程中隔天记录小鼠的生存状态及体重变化,在完成给药后观察肺顺应性和肺阻力的改变状况.留取肺泡灌洗液检测细胞转化生长因子-β(TGF-β)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)含量,观察博莱霉素引起的肺炎症改变；留取肺组织,测定肺组织中羟脯氨酸含量,并分别行HE染色和Masson's染色,观察肺组织的炎症变化和纤维化程度.结果:给予博莱霉素后,各组小鼠体重显著下降,进食减少,肺阻力升高(P＜0.05),肺泡灌洗液中TGF-β,TNF-α,IL-1β含量显著升高(P＜0.05)；灯盏生脉胶囊干预组小鼠体重下降减缓,肺阻力显著下降(P＜0.05),肺泡灌洗液中TGF-β,TNF-α,IL-1β含量明显降低(P＜0.05)；肺组织的病理切片显示肺部炎症及肺纤维化程度均有不同程度的减轻.结论:灯盏生脉胶囊能改善博莱霉素诱导的肺纤维化小鼠的虚弱状态,增强小鼠肺顺应性,降低肺阻力,抑制小鼠的肺组织炎症反应,减轻肺纤维化程度.
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| 关 键 词： | 肺纤维化 灯盏生脉胶囊 博莱霉素 炎症因子 |
| 收稿时间： | 2012/12/19 0:00:00 |
Protective Effects of Dengzhan Shengmai Capsules against Bleomycin-induced Pulmonary Fibrosis in C57BL/6 Mice |
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| ZENG Qiang,TAN Ya-xi,SUN Hong,XIAO Dan,LIU Rong,ZHU Qi,YANG Tong and ZHONG Nan-shan.Protective Effects of Dengzhan Shengmai Capsules against Bleomycin-induced Pulmonary Fibrosis in C57BL/6 Mice[J].China Journal of Experimental Traditional Medical Formulae,2013,19(11):199-203. |
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| Authors: | ZENG Qiang TAN Ya-xi SUN Hong XIAO Dan LIU Rong ZHU Qi YANG Tong and ZHONG Nan-shan |
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| Affiliation: | The State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China;The State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China;The State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China;The State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China;The State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China;The State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China;The State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China;The State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China |
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| Abstract: | |
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| Keywords: | pulmonary fibrosis Dengzhan Shengmai capsules bleomycin inflammatory cytokines |
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