不同转群时间及喷洒精油对仔猪断奶应激的影响

为探讨不同的转群时间及转群前后喷洒精油对仔猪断奶应激的影响，优化仔猪断奶转群模式，试验选择28日龄、断奶体重（7.00±0.50）kg的杜×长×大三元杂交断奶仔猪192头，随机分为4组，每组3个重复，每个重复16头。采用2×2两因素试验设计，分别探讨不同转群时间（08：00、20：00）及是否喷洒精油（喷洒、不喷洒）对断奶仔猪腹泻率、死亡率、血液生化指标、氧化指标及免疫指标的影响。结果表明：转群后1、8、24 h，各试验组仔猪血清总蛋白（TP）和白蛋白（ALB）含量差异均不显著（P>0.05）；转群后1和8 h，晚上转群组仔猪血糖（GLU）含量低于早上转群理组，喷洒精油后GLU进一步降低；仔猪转群后1、8、24 h，各试验组仔猪血清中皮质醇（COR）和丙二醛（MDA）水平随转群后时间的增加而降低。转群后1和8 h，喷洒精油的两组仔猪血清MDA水平显著低于不喷组（P<0.05），24 h后血清COR水平显著下降（P<0.05）；转群后1、8、24 h，各处理组仔猪血清中免疫球蛋白A（IgA）、免疫球蛋白M（IgM）、免疫球蛋白G（IgG）含量无显著差异（P>0.05），转群后1和24 h，早上转群但不喷洒精油组仔猪血清白细胞介素-10（IL-10）与晚上转群且不喷洒精油组无显著差异（P>0.05），而喷洒精油后，IL-10水平均显著下降（P<0.05），以晚上转群且喷洒精油组仔猪血清IL-10水平最低。综上所述，晚上转群对断奶仔猪的应激程度小于早上转群，喷洒精油能进一步缓解应激，晚上转群并喷洒精油是减少仔猪断奶转群的理想模式。

Effects of Transfer Time and Spraying Essential Oil on Weanling Stress in Weaned Piglets

This study was conducted to elucidate the effects of transfer time and spraying essential oil on weanling stress of weaned piglets so as to optimize the model of transfer,192 28-day-old Duroc pig×Large White pig×Yorkshire pig crossed weaned piglets with (7.00±0.50) kg body weight were chosen and divided into 4 groups with three repeats per group and 16 piglets per repeat.2×2 orthogonal design was used to discuss the effects of different transfer time (08:00 and 20:00) and the spraying essential oil (spraying or not spraying) on diarrhea rate,mortality,blood biochemical indexes,oxidation indexes and immune indexes of weaned piglets.The results showed that there were no significant difference of serum TP and ALB levels among the experimental groups at 1,8 and 24 h after transfer (P>0.05).The serum GLU of weaned piglets in groups transfered at 20:00 was lower than that transfered at 08:00,and that was lower after sprayed essential oil.The serum COR and MDA level were reduced with the increasing of post-transfer time.The MDA level of groups spraying essential oil were significantly decreased than that not spraying essential oil at 1 and 8 h after transfer (P<0.05),and the COR level was significantly decreased at 24 h after transfer (P<0.05).There were no significant differences of serum IgA,IgM and IgG levels among the experimental groups at 1,8 and 24 h after transfer (P>0.05).There was no significant difference of IL-10 level in groups when transfered at different time,while it was significantly decreased when sprayed essential oil (P<0.05),and that was lowest in group transfered at 20:00 and sprayed essential oil.In conclusion,the weanling stress was lower when piglets transfered in at 20:00 than at 08:00,and spraying essential oil could further relieve stress reaction,it was an ideal model to reduce piglets weaning stress when transfered in the evening and sprayed essential oil.