Skp1蛋白在非小细胞肺癌组织和外周血中的表达水平及其临床意义

目的：检测S期激酶相关蛋白1（Skp1）在非小细胞肺癌（NSCLC）组织和外周血中的表达水平并探讨其临床意义。方法：采用Western blot检测34例NSCLC组织及相应癌旁组织中Skp1蛋白的表达水平。采用组织芯片和免疫组织化学染色检测Skp1在86例NSCLC组织及相应癌旁组织中的表达情况，并分析Skp1蛋白的表达与NSCLC临床病理指标及患者预后的关系。采用酶联免疫吸附试验（ELISA）检测39例NSCLC患者及27例健康人血浆中的Skp1蛋白水平。结果：Western blot和免疫组化法分析结果显示Skp1蛋白在NSCLC组织中的表达水平均显著高于相应癌旁组织（P < 0.001）。ELISA法分析结果显示NSCLC病人血浆中的Skp1蛋白表达水平显著高于健康人群（P=0.003）。Kaplan-Meier单因素生存分析显示Skp1蛋白在NSCLC组织中的高表达与中晚期（Ⅱ+Ⅲ） NSCLC患者的不良预后显著相关（P=0.001），多因素Cox回归分析显示Skp1蛋白能够作为影响中晚期（Ⅱ+Ⅲ） NSCLC患者预后的独立危险因素。Skp1蛋白表达水平与病理类型、临床分期、分化程度、淋巴结转移等因素无显著相关关系（P > 0.05）。结论：Skp1蛋白在NSCLC患者中高表达，并且Skp1蛋白在NSCLC组织中的高表达与中晚期（Ⅱ+Ⅲ） NSCLC患者的不良预后显著相关，可作为中晚期NSCLC患者预后的独立危险因素。

Clinical significance of Skp1 protein expression in non-small cell lung cancer tissues and peripheral blood

OBJECTIVE:To detect the protein expression of Skp1(S-phase kinase-associated protein 1) in non-small cancer lung cancer (NSCLC) tissues and peripheral blood,and to explore its clinical significance.METHODS:Expressions of Skp1 protein in 34 NSCLC tissues and 34 adjacent tissues were detected by Western Blot.In addition,the expressions in 86 NSCLC tissues and 86 adjacent tissues were detected by tissue microarray and immunohistochemical staining method.The plasma expression level of Skp1 in 39 NSCLC patients and 27 healthy person was detected by ELISA (enzyme linked immunesorbent assay).The relationships between Skp1 expression with clinicopathological parameters and prognosis of NSCLC patients were analyzed.RESULTS:The expression level of Skp1 protein in NSCLC tissues was significantly higher than that in adjacent tissues by both Western blot and immunohistochemical staining methods (P < 0.01).The plasma levels in NSCLC patients were significantly higher than that in healthy person (P=0.003).The simple factors analyses show that over-expression of Skp1 protein in NSCLC tissues was significantly associated with poor prognosis in the middle to late cancer stages (Ⅱ+Ⅲ)(P=0.001),and the multiple factors Cox regression analysis showed that Skp1 protein could be used as the independent risk factors for the middle to late stages (Ⅱ+Ⅲ).The expression of Skp1 protein had no correlation with pathological type,clinical stage,pathological grade,lymph node metastasis and other factors (P > 0.05).CONCLUSION:Skp1 protein was over-expressed in NSCLC tissues and plasma from patients,and the over-expression of Skp1 protein in NSCLC tissues was significantly associated with poor prognosis in the middle to late cancer stages (Ⅱ+Ⅲ).Therefore,the over-expression can be considered for use as an independent risk factor for the middle to late stages among NSCLC patients.