| 氨基糖苷类药物双圈耐药型鲍曼不动杆菌16S rRNA甲基化酶基因研究 |
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| 引用本文： | 张晓文,邵海枫,王卫萍,李晓军.氨基糖苷类药物双圈耐药型鲍曼不动杆菌16S rRNA甲基化酶基因研究[J].中国抗生素杂志,2011,36(11). |
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| 作者姓名： | 张晓文 邵海枫 王卫萍 李晓军 |
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| 作者单位： | 南京军区南京总医院解放军临床检验医学研究所,南京,210002 |
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| 基金项目： | 南京军区联勤部卫生部2008卫生科研基金 |
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| 摘 要： | 目的 探讨在K-B法药物敏感试验中对氨基糖苷类药物双圈耐药的鲍曼不动杆菌16S rRNA甲基化酶基因携带状况以及药物诱导对该基因mRNA表达量的影响.方法 收集42株鲍曼不动杆菌,K-B法测定其药敏情况；PCR法扩增三种16S rRNA甲基化酶基因armA、rmtB及rmtC;用RT-PCR的方法分析阿米卡星诱导前后耐药基因表达量.结果 90.4％(38/42)的菌株阿米卡星药敏纸片周围呈现双圈耐药,且均检测到armA,未检测到rmtB及rmtC,其余3株为敏感,1株为普通耐药,且PCR检测耐药基因为阴性.RT-PCR结果显示经阿米卡星诱导后细菌armA基因mRNA表达量显著上升.结论 双圈耐药现象与armA基因诱导型表达有关.
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| 关 键 词： | 鲍曼不动杆菌 双圈耐药 16S rRNA甲基化酶基因 |
A study of 16S rRNA methylase on Acinetobacter baumannii double-circle resistance to aminoglycoside antibiotic |
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| Zhang Xiao-wen,Shao Hai-feng,Wang Wei-ping,Li Xiao-jun.A study of 16S rRNA methylase on Acinetobacter baumannii double-circle resistance to aminoglycoside antibiotic[J].Chinese Journal of Antibiotics,2011,36(11). |
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| Authors: | Zhang Xiao-wen Shao Hai-feng Wang Wei-ping Li Xiao-jun |
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| Affiliation: | Zhang Xiao-wen,Shao Hai-feng,Wang Wei-ping and Li Xiao-jun(PLA Institute of Clinical Laboratory Medicine,Nanjing General Hospital of Nanjing Military Command,Nanjing 210002) |
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| Abstract: | Objective To explore the presence of 16S rRNA methylase genes in Acinetobacter baumannii which is double-circle resistant to aminoglycoside antibiotic in K-B methods,and to analyze the expression levels of resistance genes mRNA among strains induced and non-induced by amikacin.Methods A total of 42 strains of Acinetobacter baumannii were collected to test the antibiotic susceptibility by disk diffusion method.The three kinds of 16S rRNA methylase genes-armA,rmtB and rmtC were amplified by PCR.The genes expr... |
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| Keywords: | Acinetobacter baumannii Double-circle 16S rRNA methylase |
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