| 阿魏酸钠对低氧性肺动脉高压大鼠肺血管平滑肌细胞Caspase-3,Bcl-2表达的影响 |
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| 引用本文： | 陈国柱,程德云,聂英.阿魏酸钠对低氧性肺动脉高压大鼠肺血管平滑肌细胞Caspase-3,Bcl-2表达的影响[J].第四军医大学学报,2009(20). |
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| 作者姓名： | 陈国柱 程德云 聂英 |
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| 作者单位： | 第三军医大学新桥医院急诊科;四川大学华西医院呼吸内科; |
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| 摘 要： | 目的:探讨阿魏酸钠(SF)对低氧性肺动脉高压大鼠肺小动脉平滑肌细胞Caspase-3,Bcl-2表达的影响.方法:将21只Wistar大鼠随机分为对照组(n=7)、低氧组(n=7)及干预组(n=7)(胃饲SF并进行低氧处理).以常压低氧复制肺动脉高压模型,右心导管插入法检测平均肺动脉压(mPAP);分别称量大鼠右心室(RV)和左心室加室间隔(LV+S)的质量,计算出右心室肥厚指数RV/(LV+S);图像分析法测量肺小动脉管壁厚度和面积,计算出肺小动脉管壁厚度指标即管壁厚度占血管外径的百分比(WT%)和管壁面积占血管总面积的百分比(WA%);进行Caspase-3,bcl-2免疫组化染色,比较各组Caspase-3和bcl-2积分光密度值(IOD).结果:①与对照组比较,低氧组大鼠平均肺动脉压(mPAP)明显升高(P<0.05)、肺小动脉WT%,WA%以及RV/(LV+S)明显增加(P<0.05).与低氧组比较,干预组大鼠平均肺动脉压(mPAP)明显降低(P<0.05)、肺小动脉WT%,WA%以及RV/(LV+S)明显减少(P<0.05).②与对照组和低氧组分别比较,干预组Caspase-3IOD值均显著增高(...
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| 关 键 词： | 低氧血症 肺动脉高压 阿魏酸钠 Caspase-3 Bcl-2 |
Effect of sodium ferulate on Caspase-3 and Bcl-2 expression levels of smooth muscle cells in pulmonary artery of hypoxic pulmonary hypertensive rats |
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| CHEN Guo-Zhu,CHENG De-Yun,NIE Ying Emergency.Effect of sodium ferulate on Caspase-3 and Bcl-2 expression levels of smooth muscle cells in pulmonary artery of hypoxic pulmonary hypertensive rats[J].Journal of the Fourth Military Medical University,2009(20). |
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| Authors: | CHEN Guo-Zhu CHENG De-Yun NIE Ying Emergency |
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| Affiliation: | CHEN Guo-Zhu1,CHENG De-Yun2,NIE Ying2 1Emergency Department,Xinqiao Hospital,Third Military Medical University,Chongqing 410037,China,2Department of Respiratory Medicine,West China Hospital,Sichuan University,Chengdu 610041 |
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| Abstract: | AIM:To investigate the impact of sodium ferulate on Caspase-3 and Bcl-2 of smooth muscle cells in pulmonary arterioles of hypoxic pulmonary hypertensive rats.METHODS:Twenty-one male Wistar rats were randomly divided into control group(n=7),hypoxic group(n=7)and sodium ferulate treated group(n=7).Mean pulmonary arterial pressure(mPAP)was measured by a right cardiac catheterization.Dry weight of right ventricle(RV),left ventricle with septum(LV+S)were determinded and RV/(LV+S)ratio was calculated.The index of... |
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| Keywords: | hypoxia pulmonary hypertension sodium ferulate Caspase-3 Bcl-2 |
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