| 无bFGF条件下简便有效建立C57BL/6J小鼠胚胎干细胞系 |
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| 引用本文： | 王晏鹏,蒋春艳,董 娟,陈 娟,高 莉,廖婷婷,崔毓桂,钱晓乔,刘嘉茵.无bFGF条件下简便有效建立C57BL/6J小鼠胚胎干细胞系[J].南京医科大学学报,2011(3):295-300. |
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| 作者姓名： | 王晏鹏 蒋春艳 董 娟 陈 娟 高 莉 廖婷婷 崔毓桂 钱晓乔 刘嘉茵 |
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| 作者单位： | 南京医科大学第一附属医院生殖医学科; |
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| 基金项目： | 江苏省医学重点学科资助(XK200702) |
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| 摘 要： | 目的:在不含bFGF的Knockout血清替代品(knockout serum replacement,KSR)的培养条件下,简便、有效地建立小鼠胚胎干细胞(embryonic stem cells,ES)系.方法:以C57BL/6J小鼠3.5天囊胚为材料,改良巴氏德管机械法分离内细胞团,于加或不加碱性成纤维细胞生长因...
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| 关 键 词： | 胚胎干细胞 碱性成纤维细胞生长因子 knockout血清替代品 C57BL/6J小鼠 |
| 收稿时间： | 2010/8/30 0:00:00 |
Efficient method to establish C57BL/6J mouse embryonic stem cell lines with bFGF-free knockout serum replacement |
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| WANG Yan-peng,JIANG Chun-yan,DONG Juan,CHEN Juan,GAO Li,LIAO Ting-ting,CUI Yu-gui,QIAN Xiao-qiao and LIU Jia-yin.Efficient method to establish C57BL/6J mouse embryonic stem cell lines with bFGF-free knockout serum replacement[J].Acta Universitatis Medicinalis Nanjing,2011(3):295-300. |
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| Authors: | WANG Yan-peng JIANG Chun-yan DONG Juan CHEN Juan GAO Li LIAO Ting-ting CUI Yu-gui QIAN Xiao-qiao and LIU Jia-yin |
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| Affiliation: | WANG Yan-peng,JIANG Chun-yan,DONG Juan,CHEN Juan,GAO Li,LIAO Ting-ting,CUI Yu-gui,QIAN Xiao-qiao,LIU Jia-yin*(Center of Clinical Reproductive Medicine,the First Affiliated Hospital of NJMU,Nanjing 210029,China) |
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| Abstract: | Objective:To establish C57BL/6J mouse embryonic stem(ES) cell lines with bFGF-free knockout serum replacement(KSR).Methods:C57BL/6J mouse blastocysts 3.5 days post coition(dpc) were collected and cultured in the medium supplemented with KSR and 1 000 U/mL LIF,with or without 10 ng/mL bFGF.The ES cell lines were identificated by morphology,alkaline phosphatase activity,karyotype,cell surface maker SSEA-1 and pluripotent markers(Oct4,Sox2 and Nanog) analysis.The differentiation ability of ES cells was analyze... |
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| Keywords: | embryonic stem cells basic fibroblast growth factor knockout serum replacement C57BL/6J mouse |
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