| 探讨Abba1的C端序列对COS7细胞产生ruffling的影响 |
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| 引用本文： | 陈燕萍,崔伯塔,郑大同,王卓璎,崔晓利,鲁 翔.探讨Abba1的C端序列对COS7细胞产生ruffling的影响[J].南京医科大学学报,2011(8):1110-1114. |
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| 作者姓名： | 陈燕萍 崔伯塔 郑大同 王卓璎 崔晓利 鲁 翔 |
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| 作者单位： | 南京医科大学第二附属医院老年科;南京医科大学第二附属医院中心实验室; |
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| 基金项目： | 江苏省卫生厅面上项目(H200912) |
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| 摘 要： | 目的:Abba1在体外实验中能介导细胞产生ruffling,其N端的IMD区域中13个赖氨酸为重要的功能氨基酸位点,但Abba1单独的IMD却不足以介导细胞产生ruffling,本研究旨在初步探讨Abba1的C端序列影响细胞产生ruffling的分子机制。方法:先构建Abba1基因重组表达质粒,分别瞬时转染COS7细胞,通过免疫荧光观察细胞形态改变,并用Western blot检测相应GTP-Rac1的蛋白水平变化。结果:过表达全长Abba1的COS7细胞形成ruffling,与对照组相比,细胞中GTP-Rac1蛋白水平升高(P<0.05);过表达Abba1-IMD和Abba1-del-357-722aa-WH2的COS7细胞不形成ruffling,与对照组相比,相应细胞中GTP-Rac1蛋白水平降低(P<0.05)。结论:Abba1的357-722aa这段区域参与介导细胞产生ruffling,而且这种影响与GTP-Rac1蛋白水平的降低有关。
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| 关 键 词： | Abba1 IMD ruffling Rac1 |
| 收稿时间： | 2011/4/27 0:00:00 |
C-terminal region affects Abba1 inducing membrane ruffling in COS7 cells |
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| CHEN Yan-ping,CUI Bo-t,ZHENG Da-tong,WANG Zhuo-ying,CUI Xiao-li and LU Xiang.C-terminal region affects Abba1 inducing membrane ruffling in COS7 cells[J].Acta Universitatis Medicinalis Nanjing,2011(8):1110-1114. |
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| Authors: | CHEN Yan-ping CUI Bo-t ZHENG Da-tong WANG Zhuo-ying CUI Xiao-li and LU Xiang |
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| Affiliation: | CHEN Yan-ping,CUI Bo-ta 1,ZHENG Da-tong 1,WANG Zhuo-ying,CUI Xiao-li,LU Xiang (Department of Geriatrics,1 Department of Central Laboratory,the Second Affilicated Hospital of NJMU,Nanjing 210011,China) |
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| Abstract: | Objective:To study whether Abba1's C-terminal region might participate in the formation of membrane ruffling.Methods:Several recombinant plasmids,such as pEGFP,pEGFP-hAbba1,pEGFP-hAbba1-IMD,pEGFP-hAbba1-del-357-722aa-WH2 were prepared.COS7 cells were transiently transfected with these plasmids,respectively.Immunofluorescent microscopy was carried out to detect the formation of membrane protrusions.And GTP-bound Rac1(GTP-Rac1)in cell lysates was determined by pull-down with GST-PAK-CRIB followed by Western b... |
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| Keywords: | Abba1 IMD ruffling Rac1 |
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