端粒酶与p53、C—myc,K—ras在肺癌组织中的表达

目的研究端粒酶活性在肺癌及癌旁组织中的表达,并探讨端粒酶的表达与p53、C-myc、K-ras之间可能存在的关系.方法采用TRAP-SYBRGreen染色法检测36例肺癌和36例癌旁组织,20例肺部良性病变组织和20例正常肺组织及A549肺腺癌细胞株端粒酶活性的表达,并利用免疫组化技术检测肺癌组织p53、C-myc、K-ras表达水平.结果83.3%的肺癌组织25.0%的癌旁组织、15.0%肺部良性病变级织表达端粒酶活性,正常肺组织未检测到端粒酶活性.端粒酶活性的表达与肺癌组织的病理类型、肿瘤大小、临床分期无相关.在30例端粒酶活性阳性的肺癌组织中有24例(80%)表达p53蛋白,18例(60%)表达C-myc蛋白,22例(73%)表达K-ras蛋白.利用Spearman分析方法分析端粒酶活性与p53、C-myc、K-ras蛋白表达水平之间的相关关系,相关系数分别为r=0.60,P＜0.01r=0.335,P＜0.01;r=0.427,P＜0.01.结论端粒酶活性的定性检测可作为肺部恶性肿瘤诊断的辅助指标之一;肺癌组织的端粒酶活性与p53、C-myc、K-ras蛋白表达水平之间存在正相关关系.

Telomerase Activity and p53 C - myc K - ras Exoression in Lung

Objective To investigate telomerase activity in lung cancer and adjacent peritumoral tissues,and possible association with expression of p53,C-myc,K-ras.Methods Thitry six cases of lung cancer and adjacent peritumoral tissues,20 lung benign lesion and normal lung tissues and A549,were examined for telomerase activity by TRAP-SYBR Green assay.p53,C-myc,K-ras were examined by immunohistochemical staining in 36 cases of lung cancer tissue specimens.Results Eighty three point three percent lung cancer specimens,25% adjacent peritumoral tissues,15% lung benign lesion were positive for telomeras activity.Telomerase activity was negative in normal lung tissues.Telomerase activity of lung cancer did not correlate with pathologic type,tumor size and clinical stage.In 30 cases of lung cancer tissue specimens with telomerase activity,p53 was overexpression in 24 cases(80%),C-myc was overexpression in 18 cases(60%),K-ras was overexpression in 22 cases(73%).Correlation was found between telomerase activity and accumulation of p53,C-myc and K-ras by Spearman assay,and the rank correlation coefficient respectively was 0.60,0.335,0.427,p<0.01 .Conclusion Qualitative analysis telomerase activity is useful in diagnosis lung neoplasms.There was correlation between telomerase activity and accumulation of p53,C-myc and K-ras in lung cancer.