Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: An in vitro system with application to the bioartificial liver |
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| Authors: | Scott L Nyberg Russell A Shatford William D Payne Wei-Shou Hu Frank B Cerra |
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| Affiliation: | (1) Department of Surgery, University of Minnesota, 55455 Minneapolis, MN, USA;(2) Department of Chemical Engineering and Materials Science, University of Minnesota, 55455 Minneapolis, MN, USA;(3) UMHC, 406 Harvard Street S.E., Box 42, 55455 Minneapolis, MN, USA |
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| Abstract: | A static culture model employing cylindrical collagen-hepatocyte gels is reported for large scale testing of conditions relevant to the three compartment hollow fiber bioartificial liver. High density hepatocyte cultivation was achieved by cell entrapment within the collagen-hepatocyte gel. Hepatocyte viability was assessed by vital staining, gel contraction, and insulin utilization. Measures of hepatocyte-specific function included albumin synthesis, ureagenesis, lidocaine biotransformation, and cholate conjugation. Although hepatocyte viability remained stable through the seven day incubation period, hepatocyte functions were not uniformly preserved. Albumin synthesis remained stable, while representative P-450 and conjugation activities decreased with time. This static culture system will facilitate the development of a hollow fiber bioartificial liver which utilizes cylindrical collagen-hepatocyte gels.Abbreviations FDA
fluorescein diacetate
- EB
ethidium bromide
- MEGX
monoethylglycinexylidide |
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| Keywords: | artificial organ bioartificial liver gel entrapment hepatocyte metabolism |
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