FocVel2基因对黄瓜枯萎病菌丝生长及毒力的影响

 由尖孢镰孢菌黄瓜专化型引起的黄瓜枯萎病是世界黄瓜生产上的一种毁灭性病害。本研究鉴定了尖孢镰孢菌黄瓜专化型丝绒蛋白的一个同源基因FocVel2,利用基因敲除和互补的方法研究该基因的功能。敲除突变体菌株ΔFocVel2出现明显的表型变化,包括菌落生长速率降低和产孢量降低,并且敲除突变株对黄瓜幼苗毒力明显减弱,回补突变体菌株能够恢复敲除突变体ΔFocVel2的所有缺陷。总之,研究结果发现丝绒蛋白基因FocVel2在菌体无性繁殖以及侵染过程中起到重要作用。

FocVel2 affects filamentous growth and virulence in Fusarium oxysporum f. sp. cucumerinum

Fusarium wilt disease, caused by Fusarium oxysporum f. sp. cucumerinum is a devastating disease in cucumber cultivation worldwide. In this study, we identified a homologue of velvet gene FocVel2 in F. oxysporum f. sp. cucumerinum. In order to determine the function of FocVel2, gene deletion and complementation experiments were performed. The mutant line ΔFocVel2 showed obvious phenotypic defects, including the reduction of mycelium growth and conidial production. Moreover, the mutant strain ΔFocVel2 showed reduced virulence in cucumber seedlings. The complementation strain was able to restore all the defects of ΔFocVel2. In sum, our results indicated that FocVel2 gene played an important role in the asexual process and pathogenicity.