Whortleberry protects kidney against the cisplatin-induced nephrotoxicity: an experimental study |
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| Authors: | Huseyin Eren Hasan Riza Aydin Levent Tumkaya Ilke Onur Kazaz Yildiray Kalkan Seher Nazli Kazaz |
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| Affiliation: | 1. Urology Department, Recep Tayyip Erdogan University School of Medicine, Rize, Turkey;2. Urology Department, Kanuni Training and Research Hospital, Medical Science University School of Medicine, Trabzon, Turkey;3. Histology and Embryology Department, Recep Tayyip Erdogan University School of Medicine, Rize, Turkey;4. Urology Department, School of Medicine, Karadeniz Technical University, Trabzon, Turkey;5. Medical Oncology Department, Kanuni Training and Research Hospital, Trabzon, Turkey |
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| Abstract: | Purpose: This study investigated the antioxidant effects of whortleberry against cisplatin-induced nephrotoxicity in rats.Material and methods: This study included 48 female Sprague–Dawley rats weighing 263.68?±?8.29?g. The rats were divided into the following six groups, with eight rats in each group: control, ethanol control, whortleberry control, cisplatin control, 16?mg/kg cisplatin +100?mg/kg whortleberry, and 16?mg/kg cisplatin +200?mg/kg whortleberry groups. Biochemical analysis was performed by measuring total oxidant status and total antioxidant status, histopathological analysis was performed by calculating proximal and distal tubule areas (μm2), and immunohistochemical analysis was performed by determining anti-Caspase-3 immunostaining. Differences among the groups were examined using one-way analysis of variance, and p?Results: Cisplatin treatment decreased the total antioxidant status and increased the total oxidant status and Caspase-3 level. Moreover, it resulted in the dilatation, vacuolization and loss of tubular epithelial cells; and glomerular degeneration and edema in the kidney tissues (p?p?Conclusions: Our results indicate that the antioxidant effects of the whortleberry decrease cisplatin-associated nephrotoxicity. |
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| Keywords: | Cisplatin nephrotoxicity kidney total antioxidant status total oxidant status whortleberry |
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