胃癌组织中增殖细胞核抗原表达及c-met基因异常的实验研究

目的探讨胃癌和相应癌旁组织中增殖细胞核抗原(PCNA)和c-met蛋白表达以及c-met基因异常扩增在胃癌中的变化规律。方法采用免疫组化抗原热修复技术分别检测58例胃癌和相应癌旁组织中c-met及PCNA蛋白的表达;半定量多聚酶链反应(PCR)技术检测c-met基因的扩增。结果58例胃癌及相应癌旁组织中均检测到c-met基因扩增,阳性率为100%,两组间c-met基因的扩增水平差异有显著性(t=9.455,P<0.01)。胃癌组c-met蛋白表达阳性率为67.24%(39/58),癌旁组织组c-met蛋白均为阴性,两组间差异有显著统计学意义(χ2=58.75,P<0.001)。58例胃癌及相应癌旁组织中PCNA标记指数(PCNALI)分别为49.3768±12.1823和14.8390±7.1847,两组间差异有显著性(t=8.805,P<0.01)。胃癌组织中c-met基因的表达和扩增与肿瘤大小、肿瘤部位、淋巴结转移、分化程度和临床分期等方面均无显著相关性。39例c-met阳性胃癌组织和19例c-met阳性胃癌组织中PCNA标记指数LI分别为48.321±7.296和30.109±5.728,统计学分析表明两组间PCNALI有显著性差异。结论胃癌组织中存在c-met基因的异常扩增和c-met及PCNA蛋白的过表达。

Study of gastric carcinoma and PCNA and c-met gene abnormality

OBJECTIVE: To explore the changed regularity of proliferating cell nuclear antigen (PCNA) and c-met gene abnormality in the oncogenic pathway of gastric carcinoma. METHODS: Semi-quantitative PCR was used to detect the amplification of c-met gene in 58 gastric carcinoma tissues and corresponding para-carcinoma tissues. Immunohistochemical staining was used to detect the expression and over expression of c-met and PCNA protein. RESULTS: The amplification of c-met was detected in 58 cases was significant statistic difference on the amplification of c-met oncogene between the two groups (t = 9.455, P < 0.01). The positive rate of c-met protein in gastric carcinoma tissues was 67.24% (39/58), which was negative in the corresponding para-carcinoma tissues. There was significant difference between the two groups (chi2 = 58.75, P < 0.001). PCNA LI of 58 gastric carcinoma tissues and the corresponding adjacent tissues were 49.3768 +/- 12.1832 and 14.8390 +/- 7.1847, respectively. The PCNA LI had a significant difference between the two groups( t = 8.805, P < 0.01) . The amplification and overexpression of c-met gene in gastric carcinoma tissues has no relationship with the tumor's size, location, lymph node metastasis, differentiation degree and deeper invasion. The amplification of c-met oncogene was related with the oncogenesis of gastric carcinoma. CONCLUSION: The abnormal expression of c-met and PCNA otein was related with the oncogenesis of gastric carcinoma.