一种快速、灵敏的血小板释放功能检测方法及其在抗血小板药物研究中的应用

本文报道了一种快速、灵敏的血小板释放功能检测方法:利用荧光素-荧光素酶在有ATP、Mg~(2+)、O_2存在时产生的生物发光素测定血小板ATP的释放量,以反映血小板的释放功能;研究了ADP、AA、胶原、凝血酶等四种诱导剂对血小板释放功能的作用,发现ADP的诱导释放能力较其他三者为弱;观察在不同剂量ADP和AA的诱导下,血小板聚集强度和释放能力之间的关系,研究了血小板数等因素对ATP释放功能测定的影响。应用该方法研究了Aspirin及活血化淤药物川芎嗪,毛冬青甲素对血小板释放功能的影响,发现Aspirin对AA诱导的释放反应有强烈的抑制作用。在以ADP诱导的释放反应中,川芎嗪的抑制作用较毛冬青甲素更为强烈。

A RAPID AND SENSITIVE METHOD FOR MONITORING PLATELET ATP RELEASE FUNCTION AND ITS APPLICATION IN ANTIPLATELET STUDIES

A rapid and sensitive method for monitoring platelet ATP release function by using biolurainescent analysis is reported here. Several factors, such as platelet number,different inducing agents (ADP, AA, thrombin, collagen) , time, etc., affecting platelet ATP release reaction were studied. At the same time, the changes of platelet aggregation intensity and ATP releasing activity at different iaducer Ievels were investigated.Effects of aspirin and two Chinese antiplatelet drugs (Ligustrazine and Ilexonin A) on platelet ATP release reactiun were observed by this method. The results showed that aspirin strongly inhibits platelet asgregation and ATP release reaction induced by AA. Ligustrazine is more potent than Ilexonin A on inhibiting platelet ATP release reaction induced by ADP.