女贞子连续超声逆流提取工艺优选

目的： 优选女贞子的连续超声逆流提取工艺。 方法： 以齐墩果酸和熊果酸总量为指标，通过正交试验考察超声频率、乙醇体积分数、料液比和温度对提取工艺的影响。采用HPLC测定齐墩果酸和熊果酸含量，流速0.33 mL·min^-1，流动相乙腈-甲醇-0.5%冰醋酸溶液（19：67：14）。 结果： 最佳提取工艺为加15倍量80%乙醇于30 kHz提取；齐墩果酸和熊果酸平均总质量分数5.66%，RSD 1.64%。 结论： 优选的连续超声逆流提取工艺操作简单、科学合理，适合于女贞子工业化大生产。

Optimization of Successive Ultrasound Countercurrent Extraction Technology of Ligustri Lucidi Fructus

Objective: To optimize successive ultrasound countercurrent extraction process of Ligustri Lucidi Fructus. Method: Taking total amount of oleanolic acid and ursolic acid as index,orthogonal design was adopted to investigate effects of ethanol concentration,ultrasonic frequency,solid-liquid ratio and temperature on extraction process of Ligustri Lucidi Fructus.ELSD was employed to determine contents of oleanolic acid and ursolic acid with mobile phase of acetonitrile-methanol-0.5% acetic acid(19:67:14) and flow rate of 0.33 mL·min^-1. Result: Optimum extraction process was that extracted with 15 times the amount of 80% ethanol at 30 kHz; under these conditions,average total amount of oleanolic acid and ursolic acid was 5.66% with RSD of 1.64%. Conclusion: This optimized extraction technology was reasonable and scientific,which was suitable for large scale production of Ligustri Lucidi Fructus.