| 响应面试验优化超声波法提取枸杞蜂花粉黄酮类化合物工艺 |
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| 引用本文： | 冉林武,闫亚美,曹有龙,曾晓雄,秦垦,魏志颖.响应面试验优化超声波法提取枸杞蜂花粉黄酮类化合物工艺[J].食品科学,2012,33(12):37-40. |
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| 作者姓名： | 冉林武 闫亚美 曹有龙 曾晓雄 秦垦 魏志颖 |
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| 作者单位： | 宁夏医科大学医学科学技术研究中心;南京农业大学食品科技学院;国家枸杞工程技术研究中心;宁夏大学农学院 |
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| 基金项目： | 宁夏回族自治区科技攻关项目(宁陕合作项目);宁夏回族自治区自然科学基金项目(NZ1065;NZ11112) |
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| 摘 要： | 通过单因素和旋转正交试验,对枸杞蜂花粉黄酮类化合物的超声波法提取工艺进行优化并建立数学回归模型。结果表明:影响枸杞蜂花粉黄酮类化合物提取的主次因素顺序为乙醇体积分数>料液比>提取时间;最佳工艺条件为乙醇体积分数75%、提取时间95min、液料比40:1(mL/g);测得枸杞蜂花粉总黄酮含量的平均值为3.03%(n=3),基本和预测值保持一致。
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| 关 键 词： | 超声波法 枸杞蜂花粉 黄酮类化合物 提取工艺 |
Optimization of Ultrasonic Extraction of Flavonoids from Wolfberry Bee Pollen Using Response Surface Methdology |
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| RAN Lin-wu,YAN Ya-mei,CAO You-long,ZENG Xiao-xiong,QIN Ken,WEI Zhi-ying.Optimization of Ultrasonic Extraction of Flavonoids from Wolfberry Bee Pollen Using Response Surface Methdology[J].Food Science,2012,33(12):37-40. |
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| Authors: | RAN Lin-wu YAN Ya-mei CAO You-long ZENG Xiao-xiong QIN Ken WEI Zhi-ying |
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| Affiliation: | 1.Research Center of Medicine Scientific Technology,Ningxia Medical University,Yinchuan 750004,China;2.College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;3.National Wolfberry Engineering Research Center,Yinchuan 750002,China;4.College of Agriculture,Ningxia University,Yinchuan 750021,China) |
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| Abstract: | On the basis of one-factor-at-a-time eperiments and central composite rotary design(CCRD) experiments,the ultrasonic extraction of flavonoids from wolfberry bee pollen was optimized and a mathematical regression model was contructed.Eththanol concentration was the most significant parameter that influences extraction efficiency,followed by material-to-liquid ratio;extraction time had the weakest influence.The optimal extraction conditions were ethanol concentration of 75%,extraction time of 95 min and material-to-liquid ratio of 1:40(g/mL).Under these extraction conditions,the average experimental extraction yield of flavonoids was 3.03%(n = 3),which was consistent with the predicted result. |
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| Keywords: | ultrasonic extraction method wolfberry bee pollen flavonoids extraction process |
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