|
|||||
|
|
| HPLC法测定藤黄中藤黄酸和新藤黄酸的含量 | |
| 引用本文： | 周安,李庆林,彭代银,吴鸿飞,范琪,王效山.HPLC法测定藤黄中藤黄酸和新藤黄酸的含量[J].中国中医药信息杂志,2008,15(8):53-54. |
| 作者姓名： | 周安 李庆林 彭代银 吴鸿飞 范琪 王效山 |
| 作者单位： | 1. 安徽中医学院科研实验中心,安徽合肥230038;安徽省现代中药研究与开发重点实验室,安徽合肥230038 2. 安徽中医学院药学院,安徽合肥230038;安徽省现代中药研究与开发重点实验室,安徽合肥230038 3. 安徽中医学院药学院,安徽合肥,230038 |
| 基金项目： | 安徽省"十一五"科技攻关项目(06013131):安徽中医学院青年科研基金 |
| 摘 要： | 目的建立藤黄中藤黄酸和新藤黄酸的含量测定方法。方法采用反相高效液相色谱法，色谱柱为Hypersil ODS C18（4．6mm×250mm，5μm）；流动相：甲醇-0．1％冰醋酸水溶液（93：7），流速1.0mL／min；检测波长为360nm，外标法定量。结果藤黄酸线性范围为2．45～49μg，回归方程Y=11334．8＋232781X（r=0．9999，n=5），平均回收率99．3％，RSD=1．02％（n=6）；新藤黄酸线性范围为2．55～51μg，回归方程Y=75197．5＋226301X（r=0．9997，n=5），平均回收率100．5％，RSD=1．48％（n=6）。结论本法精密度高，重复性好，简便、可靠，可作为藤黄的质量控制方法. |
| 关 键 词： | 高效液相色谱法 藤黄 藤黄酸 新藤黄酸 含量测定 |
Determination of the Content of Gambogic Acid and Gambogenic Acid in Gamboge by HPLC |
|
| ZHOU An,LI Qing-lin,PENG Dai-yin,et al.Determination of the Content of Gambogic Acid and Gambogenic Acid in Gamboge by HPLC[J].Chinese Journal of Information on Traditional Chinese Medicine,2008,15(8):53-54. | |
| Authors: | ZHOU An LI Qing-lin PENG Dai-yin |
| Affiliation: | ZHOU An1,3,LI Qing-lin2,PENG Dai-yin2,et al (1.The Experimental Research Center of Anhui College of TCM,Hefei 230038,China,2.The School of Materia Medica of Anhui College of TCM,3.Key Laboratory of Chinese Medicine Research , Development in Anhui Province,China) |
| Abstract: | Objective To establish a HPLC method to determinate the content of Gambogic acid and Gambogenic acid in Gamboge. Method The colum of RP-HPLC was Hypersil ODS C18 （4.6 mm×250 mm, 5 μm）. The mobile phase was consisted of methanol-water （0.1% Acetic acid） （93 ： 7）, the wavelength of detector was 360 nm, at a flow rate of 1.00 mL/min. Both were assayed with external standard method. Result The method of Gambogic acid was linear at the range of 2.45-49 μg/mL with the regression equation Y=11334.8＋232 781X（r=0.9999, n =5）, the average recovery was 99.3%, RSD was 1.02% （n=6）. The method of Gambogenic acid was linear at the range of 2.55-51 μg/mL with the regression equation Y=75 197.5＋226301X （r=0.999 7, n =5）, the average recovery was 100.5%, RSD was 1.48% n=6）. Conclusion The method is simple, accurate and reliable for the determination of the content of Gambogic acid and Gambogenic acid in Gamboge, and can be used for quality control of Gamboge. |
| Keywords: | HPLC Gamboge Gambogic acid Gambogenic acid Content determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
