Poly IC佐剂狂犬病疫苗诱导小鼠的免疫应答

目的观察PolyIC佐剂狂犬病疫苗诱导小鼠的免疫应答。方法将PolyIC佐剂狂犬病地鼠肾细胞疫苗(PHKCV+P)、人用狂犬病地鼠肾细胞疫苗(PHKCV)和中国狂犬病疫苗参考品(R)稀释后,分别于0d1次和0、7d2次腹腔注射BALB/c小鼠,另设PolyIC佐剂对照组(P)和空白对照组(N),并分别于初免后第7天和第14天处死小鼠,分离血清,检测中和抗体水平;同时取脾脏,采用ELISPOT法检测脾淋巴细胞经狂犬病疫苗刺激后分泌IFNγ的水平;另1组相同免疫的小鼠于初免后第7天和第14天用CVS毒株经脑腔攻击,观察疫苗的保护效果。结果小鼠免疫2针后,PHKCV+P组小鼠血清中和抗体水平明显高于PHKCV组;免疫1针和2针后,PHKCV+P组免疫小鼠诱生的特异性IFNγ斑点形成细胞(SFC)数均高于其他疫苗组,其保护效果明显优于PHKCV组。结论 PolyIC佐剂狂犬病疫苗可减少抗原用量,增强细胞免疫和体液免疫应答,特别是能产生早期的细胞免疫应答,从而提高传统狂犬病疫苗的保护效果。

Immune Response Induced by Rabies Vaccine Containing Poly IC Adjuvant in Mice

Objective To observe the immune response induced by rabies vaccine containing Poly IC adjuvant in mice. Methods BALB / c mice were divided into three test and two control groups, 15 for each. The mice in three test groups were injected i.p. with diluted hamster kidney cells-based rabies vaccine containing Poly IC adjuvant (PHKCV+P), hamster kidney cells-based rabies vaccine for human use(PHKCV)and national rabies vaccine reference(R)respectively, once on day 0 or twice on days 0 and 7. The mice in one control group(P)were injected with Poly IC adjuvant, while those in the other control group(N)were untreated. Five mice in each group were killed on days 7 and 14 respectively, and their sera were separated for determination of neutralizing antibody. Meanwhile, the spleens were collected for determination of secreted IFNγ levels by ELISPOT method. The rest ten mice in each group were challenged i.c. with CVS strain on days 7 and 14 to observe the protective effect of vaccine. Results The serum neutralizing antibody levels of mice immunized twice with PHKCV+P were significantly higher than those with PHKCV. However, both the specific IFNγ spot formation cell (SFC)counts of mice immunized once and twice with PHKCV+P were significantly higher than those in other groups, while the protective effects were superior to that in PHKCV group. Conclusion Rabies vaccine containing Poly IC adjuvant decreased the amount of antigen used, while enhanced the cellular and humoral immune responses especially early cellular immunity, thus induced higher protective efficacy as compared with traditional rabies vaccine.