双孢蘑菇杂交菌株As2796家系的分子遗传研究

应用PCR和凝胶电泳等技术，对双孢蘑菇杂交菌株As2796及其亲本和子代作分子遗传标记跟踪分析，结果如下: 1) 总DNA的RAPD分析表明，随着遗传代数的增加，杂种子代和出发异核体亲本间的遗传差异逐渐增大; 2) mtDNA的酶切图谱表明，亲本8213及其杂交子代具有相同的基因型，表明双孢蘑菇的mtDNA呈单亲遗传; 3) Est同工酶的PAGE图谱表明， 结合了亲本02高产特征和8213优质特征的杂交子代具有两个亲本的标记带型，证明Est同工酶标记是双孢蘑菇新菌株特性预测或鉴定的有效指标。

MOLECULAR GENETIC STUDY ON THE PEDIGREE OF HYBRID STRAIN AS2796 OF AGARICUS BISPORUS

The pedigree of the hybrid A.bisporus strain As2796 was analyzedthrough molecular genetic markers tracking. The results were as follows: 1)RAPD analysis of total DNA showed that with the addition of the number of genetic generations， the genetic differences between hybrid offspring and their original heterokaryotic parents increased. 2)The patterns of mtDNA digested by endonucleases showed that the hybrid offspring had the same mitochondrial genotype as the parent 8213， indicating that the mtDNA of A.bisporus was inherited from one of the parents. 3)PAGE patterns of esterase isozyme(Est) showed that the hybrid offsprings which combined both the characteristic of high yield of parent 02 and good quality of 8213 had the same marked bands of their parents. The Est isozyme marker was found to be an effective index of new strains' characteristic prediction or determination of A.bisporus.