| Ypel3基因在小鼠胚胎发育中的表达与调控 |
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| 引用本文： | 沈毅,韩正滨,张凤伟,顾宁,陈岩,吴琼.Ypel3基因在小鼠胚胎发育中的表达与调控[J].生物技术通讯,2011,22(5):643-646. |
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| 作者姓名： | 沈毅 韩正滨 张凤伟 顾宁 陈岩 吴琼 |
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| 作者单位： | 哈尔滨工业大学理学院生命科学与工程系,黑龙江哈尔滨,150001 |
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| 基金项目： | 国家自然科学基金，黑龙江省科技计划项目归国留学基金 |
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| 摘 要： | 目的:探讨小鼠胚胎发育过程中Ypel3基因的时空特异性表达与调控,为后续功能研究奠定基础。方法:选取胎龄(E)10.5、12.5、14.5、16.5和18.5 d的小鼠胚胎,利用荧光定量RT-PCR技术研究Ypel3基因mRNA的时序性动态表达谱;采用原位杂交技术观察Ypel3基因mRNA在胚胎发育E11.5和E15.5的空间表达谱;应用定量RT-PCR技术检测表观遗传学修饰对Ypel3基因mRNA表达丰度的影响。结果:定量RT-PCR表明该基因从胚胎发育的早中期开始表达,到出生前表达量呈逐渐升高趋势;原位杂交显示E11.5信号出现在脑和心脏中,E15.5信号在脑、舌、心、肺、胸腺、肝、肾等主要脏器中均有表达;甲基化转移酶抑制剂5-氮胞苷(5-Aza)处理的Neuro-2a(N2a)细胞中,Ypel3的表达水平未产生显著变化,而去乙酰化酶抑制剂4-苯丁酸(4-PBA)处理后该基因表达显著升高,5-Aza和4-PBA联合处理后表达水平进一步升高。结论:Ypel3基因在小鼠胚胎发育各阶段有广泛的表达,提示其具有重要作用,且该基因的表达可能受到组蛋白乙酰化的调控。
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| 关 键 词： | Ypel3基因 胚胎发育 小鼠 荧光定量PCR 原位杂交 |
Expression and Regulation of Ypel3 Gene During Mouse Embryo Development |
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| SHEN Yi,HAN Zheng-Bin,ZHANG Feng-Wei,GU Ning,CHEN Yan,WU Qiong.Expression and Regulation of Ypel3 Gene During Mouse Embryo Development[J].Letters in Biotechnology,2011,22(5):643-646. |
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| Authors: | SHEN Yi HAN Zheng-Bin ZHANG Feng-Wei GU Ning CHEN Yan WU Qiong |
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| Affiliation: | SHEN Yi,HAN Zheng-Bin,ZHANG Feng-Wei,GU Ning,CHEN Yan,WU Qiong Department of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China |
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| Abstract: | Objective: To investigate the developmental-specific expression and regulation of Ypel3 gene.Meth-ods: The temporal expression pattern of Ypel3 at embryo day(E) 10.5,12.5,14.5,16.5,18.5 were studied by flu-orescent quantitative PCR,as well as the spatial expression at E11.5 and E15.5 were studied by in situ hy-bridization.We employed the RT-PCR to investigate the influence of epigenetic modification to the expression level of Ypel3.Results: Real-time quantitative PCR showed that Ypel3 gene was expressed at ... |
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| Keywords: | Ypel3 embryo development mouse RT-PCR in situ hybridization |
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