| 诺氟沙星免疫学检测方法的建立及优化 |
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| 引用本文： | 姜金庆,李广领,王自良,刘兴友,杨雪峰,黄华国.诺氟沙星免疫学检测方法的建立及优化[J].食品科学,2011,32(21):148-151. |
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| 作者姓名： | 姜金庆 李广领 王自良 刘兴友 杨雪峰 黄华国 |
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| 作者单位： | 1.河南科技学院动物科学学院
2.动物疫病和残留物防控河南省高校工程技术研究中心 |
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| 基金项目： | 河南省高校科技创新人才支持计划资助项目(2010HASTIT026) |
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| 摘 要： | 采用优化的碳二亚胺法制备诺氟沙星(NOR)人工抗原，免疫新西兰大白兔制备多克隆抗体，建立间接竞争ELISA标准曲线。对NOR的线性检测范围为0.07～31.8ng/mL，半数抑制质量浓度(IC50)和最低检测限(LOD)分别为1.5ng/mL和0.04ng/mL。检测缓冲液中pH值和磷酸根离子浓度的最佳参数分别为7.4和10mmol/L；对甲醇和NaOH溶液(30mmol/L)的最佳调整体积分数分别为30%和10%。抗体具有广谱特异性，与环丙沙星(83.3%)、培氟沙星(78.9%)、依诺沙星(68.2%)、恩诺沙星(51.7%)和洛美沙星(41.7%)有较高的交叉反应率，可用于氟喹诺酮类药物(FQs)多残留检测方法研究。
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| 关 键 词： | 诺氟沙星 氟喹诺酮 间接竞争ELISA 多残留检测 |
Development and Optimization of Immunoassay Method for Detection of Norfloxacin Residues |
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| JIANG Jin-qing,LI Guang-ling,WANG Zi-liang,LIU Xing-you,YANG Xue-feng,HUANG Hua-guo.Development and Optimization of Immunoassay Method for Detection of Norfloxacin Residues[J].Food Science,2011,32(21):148-151. |
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| Authors: | JIANG Jin-qing LI Guang-ling WANG Zi-liang LIU Xing-you YANG Xue-feng HUANG Hua-guo |
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| Affiliation: | (1. College of Animal Science, Henan Institute of Science and Technology, Xinxiang 453003, China；2. Henan Higher Education
Engineering Technology Research Center for Animal Diseases Control and Residues Supervison, Xinxiang 453003, China) |
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| Abstract: | Modified EDC method was employed to synthesize norfloxacin(NOR) artificial antigen and New Zealand rabbits were immunized for anti-NOR polyclonal antibody(pAb) production.An indirect competitive ELISA standard curve was established.The developed assay was sensitive and had a linear range from 0.07 to 31.8 ng/mL(R2 = 0.9627),with IC50 and LOD of respectively 1.5 ng/mL and 0.04 ng/mL.The optimal pH and phosphate ion concentration in assay buffer were determined to be 7.4 mmol/L and 10 mmol/L,respectively,and ... |
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| Keywords: | norfloxacin(NOR) fluoroquinolone(FQs) indirect competitive ELISA multi-residue detection |
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