| 双波长法测定木薯淀粉中直链和支链淀粉的含量 |
| |
| 引用本文： | 石海信,郝媛媛,方怀义,熊拯.双波长法测定木薯淀粉中直链和支链淀粉的含量[J].食品科学,2011,32(21):123-127. |
| |
| 作者姓名： | 石海信 郝媛媛 方怀义 熊拯 |
| |
| 作者单位： | 钦州学院化学化工学院 |
| |
| 基金项目： | 广西科学基金资助项目(桂科自0991274); 广西高校重点实验室资助项目(HYSYS-YB07) |
| |
| 摘 要： | 目的：分离纯化木薯淀粉中的直链和支链淀粉，建立同时测定木薯淀粉中直链与支链淀粉含量的双波长法。方法：采用正丁醇结晶法分离纯化直链和支链淀粉，蓝值比较法表征直链与支链淀粉的纯度；根据双波长法原理，分别在测定波长624、538nm，参比波长440、750nm处测定木薯淀粉中直链与支链淀粉含量。结果：分离纯化得到的直链与支链淀粉蓝值分别为0.979和0.144，分别落在0.8～1.2与0.08～0.22范围内，表明纯化后木薯直链与支链淀粉的纯度较高；直链淀粉在0～80mg/L质量浓度范围内其碘复合物与吸光度呈线性关系(r＝0.9992)，支链淀粉在0～220mg/L质量浓度范围内其碘复合物与吸光度呈线性关系(r＝0.9995)。结论：正丁醇结晶法能有效地分离木薯直链与支链淀粉；双波长法操作快速、准确，无需分离即可同时测定木薯淀粉样品中直链和支链淀粉含量。
|
| 关 键 词： | 直链淀粉 支链淀粉 分离 正丁醇结晶法 双波长法 |
Separation and Purification of Amylose and Amylopectin from Cassava Starch and Content Determination by Dual-wavelength Spectrophotometry |
| |
| SHI Hai-xin,HAO Yuan-yuan,FANG Huai-yi,XIONG Zheng.Separation and Purification of Amylose and Amylopectin from Cassava Starch and Content Determination by Dual-wavelength Spectrophotometry[J].Food Science,2011,32(21):123-127. |
| |
| Authors: | SHI Hai-xin HAO Yuan-yuan FANG Huai-yi XIONG Zheng |
| |
| Affiliation: | (College of Chemistry and Chemical Engineering, Qinzhou University, Qinzhou 535000, China) |
| |
| Abstract: | Objective: To separate and purify amylose and amylopectin from cassava starch and to establish a dual-wavelength spectrophotometric method for determining their contents.Methods: The amylose and amylopectin in cassava starch were separated by n-butanol crystallization method.The purity of amylose and amylopectin was characterized by blue value of starch-iodine complex.According to the principle of dual-wavelength spectrophotometry method,the contents of amylose and amylopectin in cassava starch were determi... |
| |
| Keywords: | amylose amylopectin separation n-butanol crystallization dual-wavelength spectrophotometry |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
