| 云南栽培种及野生种芋头的AFLP指纹分析 |
| |
| 引用本文： | 李婉莎,刘德团,胡向阳.云南栽培种及野生种芋头的AFLP指纹分析[J].生物技术通讯,2011,22(6):855-858. |
| |
| 作者姓名： | 李婉莎 刘德团 胡向阳 |
| |
| 作者单位： | 中国科学院昆明植物研究所,云南昆明,650204 |
| |
| 基金项目： | 国家自然科学基金面上项目，中国科学院项目百人计划 |
| |
| 摘 要： | 目的:分析云南芋头种质资源遗传多样性。方法:应用扩增片段长度多态性(AFLP)指纹技术,用3对AFLP引物对采集自云南省的9份芋头栽培品种及1份野生品种进行研究,分离AFLP多态性条带。结果:共分离到60个AFLP多态性条带,AFLP多态位点百分率为96.77%,云南芋头种质资源在DNA分子水平上表现出丰富的遗传多样性。聚类分析将10份芋头品种分为2组,遗传距离为0.101～0.908。结论:AFLP指纹技术是筛选品种间差异基因的有效方法,研究结果为云南省芋头品种鉴定、遗传相关性分析、特殊功能基因的分离等工作提供了一定的理论基础。
|
| 关 键 词： | 芋头 扩增片段长度多态性 指纹分析 遗传多样性 |
AFLP Finger Analysis Between Cultivars and Wild Type of Taros (Colocasia esculenta) from Yunnan,China |
| |
| LI Wan-Sha,LIU De-Tuan,HU Xiang-Yang.AFLP Finger Analysis Between Cultivars and Wild Type of Taros (Colocasia esculenta) from Yunnan,China[J].Letters in Biotechnology,2011,22(6):855-858. |
| |
| Authors: | LI Wan-Sha LIU De-Tuan HU Xiang-Yang |
| |
| Affiliation: | LI Wan-Sha,LIU De-Tuan,HU Xiang-Yang Kunming Institute of Botany,Chinese Academy of Science,Kunming 650204,China |
| |
| Abstract: | Objective: To identify genetic diversities of taro germplasms in Yunnan province,China.Methods: The amplified fragmeng length polymorphism(AFLP) fingerprinting technology was used to analyze nine cultivars and one wild taro sampled in Yunnan province and to screen AFLP polymorphic bands.Results: 60 AFLP polymorphic bands were screened through three pairs of AFLP primes among these taros varieties and the ratio of polymorphic loci was 96.77%.It showed rich genetic diversities in taro germplasms of Yunnan pro... |
| |
| Keywords: | amplified fragmeng length polymorphism finger analysis genetic diversity taro |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
