慢性肾小球肾炎患者治疗前后血清CRP和VEGF浓度变化及临床意义

目的：研究慢性肾小球肾炎（CNG）中血清C反应蛋白（CRP）和血管内皮生长因子（VEGF）的浓度变化及其临床意义。方法：采用ELISA法测定35例正常对照组与41例慢性肾小球肾炎患者治疗前后血清IL-6和VEGF的浓度,同时放射免疫分析法测定血清TNF-α浓度,免疫比浊法测定血清CRP与尿Alb浓度。结果：①治疗前后CNG患者血清中IL-6、TNF-α和CRP较正常对照组均显著升高（P〈0.05或P〈0.01）,但治疗后IL-6、TNF-α和CRP水平显著低于治疗前（P〈0.01）,且血清CRP与IL-6和TNF-α呈正相关（P〈0.01）。②治疗后,CNG患者血清VEGF水平与尿Alb含量较治疗前明显降低（P〈0.05或P〈0.01）,仍显著高于正常对照组（P〈0.05或P〈0.01）,且血清VEGF与尿Alb水平呈正相关（P〈0.01）。结论：CRP、IL-6和TNF-α参与了CNG患者慢性炎症反应,VEGF则与蛋白尿的产生密切相关,治疗前后血清CRP和VEGF检测对于慢性肾小球肾炎的病情了解及临床疗效评估均具有重要的临床价值。

Clinical significance of detection of serum CRP and VEGF levels before and after treatment in patients with chronic glomerular nephritis

Objective：To study the level changes of serum C reactive protein（CRP） and vascular endothelial growth factor（VEGF）,and the clinical significance of detection of CRP and VEGF.Methods：Serum IL-6 and VEGF were determined by ELISA assay in 41 patients with CNG and 35 normal people.The radio immunoassay was applied to detect serum TNF-α,and immunoturbidimetry to detect serum CRP and urine Alb.Results：① Serum IL-6,TNF-α and CRP in patients with CNG were higher than control group（P〈0.01）,and Serum IL-6,TNF-α and CRP in patients after treatment were significantly lower than before treatment（P〈0.01）.There were positive correlation between serum IL-6,TNF-α and CRP levels（P〈0.01）.②After treatment,the serum CRP and urine Alb significantly decreased（P〈0.01 or P〈0.05）,but still higher than control group（P〈0.01）.The serum VEGF levels were positively correlated with urine Alb（P〈0.01）.Conclusion：IL-6,TNF-α,CRP may be involved in chronic inflammatory process in CNG,and VEGF has close relation-ship with proteinuria.Detection of serum CRP and VEGF before and after treatment plays important role in understanding the pathoge-netic condition and observing efficacy of patients with CNG.