罗丹明B—Fe2＋-H2O2光度法测定两种中药抗氧化活性

目的研究生首乌和当归两种中药的抗氧化活性。方法采用Fenton反应产生可使罗丹明B的吸光度发生变化的羟基自由基，加入中药的水提物可部分清除溶液中的羟基自由基，通过测定加入中药前后吸光度差值（△4值）的变化测定中药的抗氧化活性。结果两种中药的水提物对羟基自由基的清除率分别为59．2％和52．5％。结论生首乌与当归的水提物均有较好的抗氧化活性。

Evaluation of Antioxidation Activities of Two Chinese Medicinal Herbs bv Rhodamine B-Fe2+-H2O2 Photometry

Objective To evaluate the antioxidation activities of two Chinese herbs, polygonum muhiflorum and Angelica sinensis. Methods The Fenton reaction was adopted for producing hydroxyl free radicals, which could change Rhodamine B light absorbance. Water- soluble extracts added the traditional Chinese medicinal herbs could remove hydroxyl radicals in solution. The antioxidation activity was detecting by monitor- ing the change （AA value） before and after adding the traditional Chinese medicinal herbal extracts. Results Using this indirect method for monitoring the antioxidation activities, the water-soluble extracts of these two herbs had better antioxidation activities, in which the removing rate of hydroxyl free radicals was 59.2% and 52.5% respectively. Conclusion Po｜ygonum muhiflorum and the aqueous extract of angelica sinensis have good antioxidant activity.