2015年江苏省网店自制食品中食源性致病菌的 监测分析

目的 监测江苏省网店自制食品食源性致病菌的污染状况。了解金黄色葡萄球菌产毒及耐药特点。方法 GB4789《食品安全国家标准 食品微生物学检验》方法,选取无锡、常州、盐城等3个监测点,采集我省范围内的网店自制食品共214份,进行沙门氏菌、金黄色葡萄球菌、单增李斯特菌的检验。对分离得到的金黄色葡萄球菌进行葡萄球菌肠毒素检验和药物敏感性试验。结果 共检出食源性致病菌13株,总检出率为6.1%。其中金黄色葡萄球菌9株单增李斯特菌4株,未检出沙门菌。3份样品金黄色葡萄球菌污染超出国家食品限量值。9株金黄色葡萄球菌中6株产生葡萄球菌肠毒素。结论 江苏地区网店自制食品存在食源性致病菌污染状况。其中金黄色葡萄球菌污染比较严重,产肠毒素比例高,且对多种抗生素耐药,对青霉素产生普遍耐药, 对四环素、万古霉素和利福平耐药率较高, 对苯唑西林、环丙沙星、奎奴普丁/达福普汀和呋喃妥英等耐药率相对较低；对耐莫西沙星、利奈唑胺、替加环素和甲氧苄氨嘧啶/磺胺甲恶唑有较好的敏感性。

Monitoring and analysis of foodborne pathogens in homemade food from online-shops in Jiangsu province in 2015

Objective: To monitor the online-shopped homemade food bacterial contamination status; to understand the the characteristic of Staphylococcal enterotoxins and antibiotic resistance.Method: Using GB4789 national food safety standard, take 214 homemade food samples through Internet from 3 cities in Jiangsu Province, detect Salmonella, Staphylococcus aureus and Listeria monocytogenes. The isolated Staphylococcus aureus were tested for Staphylococcal enterotoxins and antimicrobial susptiblity. Results: 13 bacteria(9 Staphylococcus aureus and 4 Listeria monocytogenes) strains were detected, the positive rate was 6.1%. Staphylococcus aureus of 3 samples were beyond the threshold of national food safety standard. 6 stains of 9 isolated Staphylococcus aureus were positive for Staphylococcal enterotoxins.