| 肿瘤相关候选基因在舌鳞癌中的筛选 |
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| 引用本文： | 张胜,蒋飞荣,周文,冯湘玲,吴汉江,凌天牖.肿瘤相关候选基因在舌鳞癌中的筛选[J].生物磁学,2009(20):3877-3881. |
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| 作者姓名： | 张胜 蒋飞荣 周文 冯湘玲 吴汉江 凌天牖 |
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| 作者单位： | [1]中南大学湘雅二医院口腔中心 [2]广西中医学院药理教研室 [3]中南大学肿瘤研究所 |
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| 基金项目： | 基金项目：湖南省卫生厅科研基金课题（B2007-052）,长沙市科技局科技计划（K0802135-31） |
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| 摘 要： | 目的：寻找舌癌特异性相关基因,阐明舌癌发病的分子机制,为舌癌的诊断、治疗和预后提供分子靶标。方法：采用半定量RT-PCR方法检测了所选9个肿瘤相关基因DLC1、ANXA1、BCAT1、KRAS2、KCNJ8、LDHB、DNM1L、ETNK1、PTX1在舌鳞癌中的表达情况。结果：DLC1、ANXA1、LDHB、DNM1L、ETNK1、PTX16个基因在舌鳞癌组织与配对正常组织中无明显表达差异;而BCAT1、KRAS2、KCNJ8在舌鳞癌组织中表达上调,上调频率分别为45%（9/20）、50%（10/20）和33.3%（4/12）。结论：BCAT1、KRAS2和KCNJ8参与了舌鳞癌的发生发展,也为进一步在舌鳞癌中分析3个基因的功能提供实验依据。
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| 关 键 词： | 舌鳞癌 肿瘤相关基因 表达 RT-PCR |
Screening of Tongue Squamous Cell Carcinoma Correlative Candidate Genes |
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| Affiliation: | ZHANG Sheng, JIANG Fei-ron, ZHOU Wen, HONG Xiang-ling, WU Han-jiang, LING Tian-yong ( 1 Department ofstomatology, the Second Xiangya Hospital, Central South University, Changsha 41001 I, China 2 Department ofPharmocology, Guangxi Traditlbnal Chinese Medical University, ？Canning 530001, China; 3 Cancer Research Institute, Central South University Changsha 410078, China) |
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| Abstract: | Objective： To explore its pathogenesis of tongue squamous cell carcinoma and find new and effective treatment methods. Methods： This study used semi-quantitative RT-PCR method to detect the expression of 9 selected tumor-related genesDLC1, ANXA1,BCAT1,KRAS2,KCNJ8,LDHB,DNM1L, ETNK1,PTXI,in 20 tongue squamous cell carcinoma cases. Results： Results show that 6 gene, DLC1, ANXA1, LDHB, DNM1L, ETNK1, PTX1, in tongue squamous cell carcinofna and matched tissue samples had no significant expression difference, but BCAT1, KRAS2, KCNJ8 expression in tongue squamous cell carcinoma increased, the frequencies were 45% （9/20）, 50% （10/20） and 33.3% （4/12）. Conclusions： This might suggested that BCAT1, KRAS2, and KCNJ8 was involved in the occurrence and development in tongue squamous cell carcinoma, but also provided the experimental basis for further analysis of gene function in tongue squamous cell carcinoma. |
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| Keywords: | tongue squamous cell carcinoma relative gene expression RT-PCR |
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