用重组SAG1抗原检测弓形虫抗体

目的探讨重组SAG1抗原对弓形虫IgG和IgM抗体的检测效果。方法用rSAG1作抗原建立免疫印迹方法（rSAG1-WB），与玻片虫体过氧化物酶免疫染色试验（TSHE）平行检测不同来源血清。结果15例病原学检查阳性小鼠血清和5例免疫兔血清的IgG抗体均为阳性，30例正常小鼠血清和10例正常兔血清均未出现阳性反应。rSAG1-WB检测可疑弓形虫病患者血清阳性率为60．3％（38／63），献血员血清阳性率为6％（3／50），与TSHE检测结果（65．1％和4％）均无统计学差异（P〉0．05）。1例IgM强阳性血清和13例IgM弱阳性血清在Western—blot检测中分别出现相应的强阳性与弱阳性反应，50例献血员血清均未出现IgM阳性反应，结果与TSHE一致。结论rSAG1-WB检测弓形虫IgG和IgM抗体均具有高度的敏感性和特异性．与TSHE的符合率高。

Detection of Antibodies to Toxoplasma gondii by Recombinant SAG1 Antigen

Objective To evaluate the applicability of the recombinant major surface membrane antigen （rSAG1） in the detection of IgG and IgM antibodies to Toxoplasma gondii. Method rSAG1 of T.gondii was used in the Western blotting （WB） analysis of anti-SAG1 IgG and IgM in sera from different sources. The results were compared with the Toxoplasma-slide HRP-enzyme immunostaining （TSHE） method. Result Anti-SAG1 IgG was detected in sera from 15 mice infected with T.gondii and 5 rabbits immunized with soluble antigen of tachyzoites. None of the serum from 30 normal mice and 10 normal rabbits showed positive reaction. The seroprevalenee was 60.3% （38/63） for the suspected patients and 6% （3/50） for the blood donors tested by rSAG1-WB. The result was not significantly different from TSHE（65.1% and 4% respectively, P〉0.05）. For the detection of IgM antibodies, rSAG1- WB showed the same result as TSHE. IgM was detected in all 13 samples by the rSAG1-WB, and no positive reaction was detected in samples from 50 blood donors. Conclusion Both rSAG1-WB and TSHE are sensitive and specific for IgG and IgM antibodies to T. gondii.