大鼠三叉神经尾侧亚核内N-甲基-D-天冬氨酸型受体与磷酸激活的谷氨酰氨酶和谷氨酸的分布

方法:免疫细胞化学ABC法;目的:调查N-甲基-D-天冬氨酸型受体、磷酸激活的谷氨酰氨酶和谷氨酸在大鼠三叉神经尾侧亚核内的分布及其匹配关系.结果:N-甲基-D-天冬氨酸型受体1和2A/B亚单位免疫阳性胞体和纤维密集分布于大鼠三叉神经尾侧亚核的浅层(Ⅰ、Ⅱ层),磷酸激活的谷氨酰氨酶和谷氨酸免疫阳性胞体分布于三叉神经尾侧亚核各层,尤以浅层密集.磷酸激活的谷氨酸氨酶和谷氨酸阳性纤维及终未样结构主要分布于三叉神经尾侧亚核浅层.以上结果表明三叉神经尾侧亚核内磷酸激活的谷氨酰氨酶和谷氨酸免疫阳性纤维及终末样结构的分布与N-甲基-D-天冬氨酸型受体阳性胞体和纤维的分布在总体上是相互匹配的.结论:三叉神经尾侧亚核内的谷氨酸可能通过作用于N-甲基-D-天冬氨酸型受体的不同亚单位而发挥多种生理功能.

DISTRIBUTION OF NMDA RECEPTOR, PHOSPHATE ACTIVATED-GLUTAMINASE AND GLUTAMATE IN RAT CAUDAL TRIGEMINAL SUBNUCLEUS

The distribution and match relationship of NMDA receptor (NMDAR), phosphate activated-glutaminase(PAG) and glutamate(Glu) in the caudal trigeminal subnucleus(Vc) in rats were in-vestigated by using immunocytochemistry ABC method. The results were as follows: 1. Numerous NMDAR1-, NMDAR2A/B-immunopositive perikarya and fibers were densely dis-tributed in the superficial layer of Vc. PAG-and Glu-immunopositive perikarya were distributed in every layer, especially in the superficial layer of Vc. PAG-and Glu-immunopositive fibers and terminal-like structures were concentrated in the superficial layer of Vc. 2. The distribution of Glu-and PAG-immunopositive fibers and terminal-like structures were generally consistent with that of NMDAR-like immunopositive perikarya and fibers. 3. Glu might affect many kinds of physiological functions by acting on different NMDAR subunits in Vc.