乳腺癌组织HIF-1α和Sema 4D表达及其与淋巴管密度和各临床病理指标的关系

目的通过检测HIF-1α和Sema 4D在乳腺癌组织表达,探讨其与淋巴管密度（LVD）和临床病理指标的关系。方法采用免疫组化SP法检测110例乳腺癌组织标本HIF-1α和Sema 4D表达,检测微淋巴管密度（LVD）,依据不同临床病理特征分组并进行组间比较,并与乳腺纤维腺瘤组织标本对照。结果乳腺癌组织HIF-1α和Sema 4D阳性表达率分别为72.72%（80/110）和77.27%（85/110）;HIF-1α与Sema 4D表达呈正相关性（r=0.691,P〈0.01）。乳腺癌组织LVD显著高于纤维腺瘤组（P〈0.01）,淋巴结转移组LVD显著高于无淋巴结转移组（P〈0.01）;p TNMⅠ~Ⅱ期组、淋巴结无转移组癌组织HIF-1α和Sema 4D表达阳性率明显低于p TNMⅢ期组、有淋巴结转移组（P〈0.05,P〈0.01）,不同年龄、组织学分级、肿块大小组之间差异无统计学意义（P〉0.05）。结论乳腺癌组织HIF-1α、Sema 4D高表达,可能与淋巴管的增生、转移侵袭有密切关系。

Expressions of HIF-IoL and Semaphorin 4D Protein in Human Breast Carcinoma and Their Relationship with Lymphatic Vessel Density and Clinical Pathological Indicators

Objective To detect expressions of HIF-1α and semaphorin（Sema） 4D in breast carcinoma and explore their correlation with lymphatic vessel density （LVD） and clinical pathological indicators. Methods The expression of HIF-1α and Sema 4D in 110 specimens of breast carcinoma were detected by immunohistochemical SP method and LVD in all specimens was measured. Different clinical pathological features were compared and breast fibroadenoma tissues were used as control group. Results The positive rates of HIF-1α and Sema 4D expression were 72.72%（80/110） and 77.27%（85/110）, respectively. HIF-1α expression were positively correlated with Sema4D expression （r=0.691, P〈0.01）. The LVD were significantly higher in breast carcinoma group than that in breast fibroadenoma group, higher in lymph node metastasis group than that in no lymph node metastasis group（P〈 0.01 ）. The positive rates of HIF-1α and Sema 4D in pTNM Ⅰ- Ⅱ group and no lymph node metastasis group were significantly lower than those in pTNM Ⅲ group and lymph node metastasis group（P〈0.05, P〈0.01 ）. No significant difference in the positive rates of HIF-1α and Sema 4D was found among different age groups, histological differentiation groups and tumor size groups（P〉0.05）. Conclusion The high expression of HIF-1α and Sema 4D in breast carcinoma may have close relationship with lymphatic hyperplasia, lymph node metastasis, and invasion.