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New Nonnucleoside Substrates for Terminal Deoxynucleotidyl Transferase: Synthesis and Dependence of Substrate Properties on Structure
Authors:Email author" target="_blank">A?L?KhandazhinskayaEmail author  M?K?Kukhanova  M?V?Jasko
Affiliation:(1) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 119991, Russia
Abstract:N-(9-Fluorenylmethoxycarbonyl)-ω-aminoalkyl-, N-(9-fluorenylmethoxycarbonyl)-8-amino-3,6-dioxaoctyl, and N-(9-fluorenylmethoxycarbonyl)-6-aminohexanoyl]-2-aminoethyl triphosphates were synthesized. All of them were shown to be the substrates of the calf thymus terminal deoxynucleotidyl transferase. Their substrate properties depend on the length and structure of the linker between the 9-fluorenylmethoxycarbonyl and triphosphate moieties.__________Translated from Bioorganicheskaya Khimiya, Vol. 31, No. 4, 2005, pp. 394–398.Original Russian Text Copyright © 2005 by Khandazhinskaya, Kukhanova, Jasko.
Keywords:nucleoside triphosphate analogues  substrate properties  terminal deoxynucleotidyl transferase
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