| 不同类型油菜EST-SSR标记的通用性及其应用研究 |
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| 引用本文： | 姜蔚,张泽华,赵惠贤,胡胜武.不同类型油菜EST-SSR标记的通用性及其应用研究[J].西北植物学报,2009,29(11). |
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| 作者姓名： | 姜蔚 张泽华 赵惠贤 胡胜武 |
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| 作者单位： | 1. 西北农林科技大学,生命科学学院,陕西杨陵,712100
2. 西北农林科技大学,农学院,陕西杨陵,712100 |
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| 基金项目： | 国家油菜产业技术体系化杀岗位科学家项目 |
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| 摘 要： | 以10个甘蓝型油菜、11个芥菜型油菜和7个白菜型油菜品种为材料,选用已报道的14对白菜EST-SSR引物和8对油菜EST-SSR引物,探索其在3种类型油菜中的通用性,并利用筛选出的在3类油菜中有通用性和多态性的EST-SSR标记对供试油菜品种进行聚类分析.结果表明:(1)选用的14对白菜EST-SSR引物和8对油菜EST-SSR引物,在供试的3种类型油菜中都有扩增,完全可用.(2)在白菜型、甘蓝型和芥菜型油菜品种中扩增显示出多态性的引物数分别为18、16 和14对,其中有10对引物在3种类型油菜品种间扩增产物具有多态性.(3)利用这10对扩增多态性EST-SSR引物对供试油菜品种聚类分析,结果显示,在遗传相似系数为0.67时,3种类型油菜品种分别独自聚为一大类,表明开发和建立不同类型油菜间可通用的EST-SSR标记是可行且有应用价值的.
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| 关 键 词： | 油菜 EST-SSR标记 通用性 |
Transferability of EST-SSR Markers among Three Types of Oilseed Rape and Its Application |
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| JIANG Wei,ZHANG Ze-hua,ZHAO Hui-xian,HU Sheng-wu.Transferability of EST-SSR Markers among Three Types of Oilseed Rape and Its Application[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(11). |
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| Authors: | JIANG Wei ZHANG Ze-hua ZHAO Hui-xian HU Sheng-wu |
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| Affiliation: | JIANG Wei1,ZHANG Ze-hua1,ZHAO Hui-xian1*,HU Sheng-wu2 (1 College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China,2 College of Agronomy,China) |
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| Abstract: | Fourteen pairs of EST-SSR primer designed for Chinese cabbage and 8 pairs for oilseed rape were selected to conduct PCR amplification and analyze the ploymorphism of the PCR products of 10 accessions of Brassica napus,11 Braasica juncea and 7 Brassica campestris.The purpose of this study was to test the transferability of EST-SSR markers among the three types of oilseed rape.The results showed that the 14 pairs of EST-SSR primer for Chinese cabbage and the 8 pairs for oilseed rape had amplified products in ... |
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| Keywords: | oliseed rape EST-SSR marker transferability |
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