尖锐温疣患者有乳头瘤病毒6,11型的检测及L1基因的序 …

目的 检测尖锐湿疣患者组织中人乳头瘤病毒（ＨＰＶ）６型和１１型的感染率,分析Ｌ１晚期基因序列及其蛋白质的氨基酸序列,为基因工程疫苗的研究提供依据。方法 采用ＰＣＲ方法测定北京和锦州两地区３４例尖锐湿疣患者病损组织中ＨＰＶ６型及１１型的感染率,并扩增Ｌ１晚期基因,构建重组测序质粒,双脱氧法测序观察其变异状况。结果 ＨＰＶ１１型感染率为７３．５％（２５／３４）,６型感染率为４４．１％（１５／３４）,其

Assay and L1 gene sequence analysis of human papillomavirus type 6 and 11 in condylomata acuminata

OBJECTIVE: To determinate the prevalence of HPV type 6, 11 in condylomata acuminata, and analyze the sequence of L1 gene and its deduced L1 protein. METHODS: PCR was used to amplify DNA sequences between E6 and E7 of HPV type 6 and 11. The PCR products were identified by the fragment length cleaved with restriction enzyme RsaI. L1 genes were amplified with high fidelity Taq, and cloned DNA sequences were detected by the dideoxynucleotide method. RESULTS: In 34 examples of condylomata acuminata, HPV11 was found in 25 examples (73.5%), HPV6 in 15(44.1%), including HPV6 + HPV11 in 6(17.6%). In comparison with the prototypes there were 7-8 point mutations in HPV11 L1 gene and 3 in HPV6L1 gene. L1 encoded protein changes showed up only in 1-3 amino acids in the former cases. CONCLUSION: Most of patients tested with condylomata acuminata are mainly infected by HPV11, the rest of them by HPV6 or by co-infection of two mixex types. Sequence analysis of L1 genes indicated that mutations were found in both HPV6 and 11, whereas deduced amino acid mutations in L1 protein were limited in HPV11 only.