中国拟青霉液体深层发酵条件的优化

中国拟青霉(Paecilomyces sinensis sp nov,PS)属于冬虫夏草中的一个无性型菌株,研究表明其与冬虫夏草在生物活性成分方面十分接近。该文通过单因素和正交试验对中国拟青霉液体深层发酵进行条件优化。对中国拟青霉进行发酵培养,选取菌丝体生物量为指标,确定碳源、氮源和无机盐的最佳比例和最优发酵条件。中国拟青霉液体深层发酵的最佳培养基配比为:玉米粉4.0%、葡萄糖1.5%、黄豆粉2.0%、酵母粉0.3%、KH2PO40.2%、MgSO40.1%、CaSO40.1%、ZnSO40.03%。优化后的菌丝体生物量比优化前提高了1.22倍,而胞内糖肽产量与优化前相比提高了54.0%。

The Optimization of Liquid Submerged Fermentation Conditions of Paecilomyces sinensis

Paecilomyces sinensis sp nov is an anamorphic strain isolated from Cordyceps sinensis,which is traditional Chinese medicine.It exhibits high similarity to cordyceps sinensis in bioactive components.In this study,the conditions of submerged fermentation were optimized through single factor screening test and orthogonal experiments.The optimal ratio of carbon source,nitrogen source and inorganic salt was identified,accessing to the mycelia biomass.The optimal medium ratio of the submerged fermentation was as follows:corn flour 4.0%,glucose 1.5%,soybean meal 2.0%,yeast extrct 0.3%,KH2PO40.2%,MgSO40.1%,CaSO40.1%,ZnSO40.03%.The biomass of mycelium after optimization was 1.22 times higher than that before.The yield of intracellular glycopeptide was increased by 54.0%compared with that before.