慢性心功能不全时经主动脉移植干细胞的可行性研究

心肌梗死或/和慢性心功能不全时,心脏干细胞移植是一项颇有应用前景的新的治疗技术。但是,对于非缺血性心脏病所致的慢性心功能不全,目前所采用的移植路径有一定的局限性。本试验拟研究慢性心功能不全时,封堵主动脉窦上部经主动脉根部移植干细胞是否可行。阿霉素所致的慢性心功能不全兔模型,采用双球囊封堵主动脉窦上部经主动脉根部自体移植骨髓单核细胞,4周后评价心功能。在封堵主动脉窦上部经主动脉根部移植干细胞时,无1例因移植手术死亡。双球囊充盈时造影显示,主动脉窦显影良好,大部分情况下,左、右冠状动脉可显影。骨髓单核细胞移植4周后,左室射血分数改善明显(P=0 .0 34)。所以,慢性心功能不全时,封堵主动脉窦上部经主动脉根部移植干细胞安全可行。

Feasibility of Stem Cells Transplantation Through Aorta in Adriamycin-induced Heart Failure

Stem cells transplantation is a promising strategy for tre ating myocard ial infarction and/or chronic heart failure; however, with respect to nonischemi c heart failu re, there are some limitations inherent in the current methods of transplantatio n. In this study, we investigated the feasibility of a novel method, i.e. trans plantation through the root of aorta when the ascending aorta occluded above the sinus aortae. Jap anese white ears rabbits were used as chronic heart failure models by intravenou s injection of adriamycin. Autologous bone marrow mononuclear cells (MNC) were i n fused into the root of aorta when the ascending aorta was occluded by a couple o f balloons above the sinus aortae. After 4 weeks, ejection fraction was signific antly improved in MNC group. In conclusion, we have developed a unique method fo r efficien t and safe cell transplantation based on infusion in aorta. This method, potenti ally suitable for nonischemic heart failure and could be used to achieve even and global supply of cells in heart.