次氯酸钠溶液对临床常见细菌的敏感性观察

目的通过试验观察几种常见细菌对次氯酸钠消毒剂的敏感性,为临床使用含氯消毒剂提供理论依据。方法配制0～50mg/L 6种不同浓度的次氯酸钠溶液,将一定量的金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、肺炎克雷伯菌、鲍氏不动杆菌、嗜热脂肪芽胞杆菌悬液与不同浓度次氯酸钠溶液混合作用5min,设6个试验组,按常规方法接种培养,观察灭菌效果。结果对照组(0mg/L)细菌生长良好,菌落数平均为1123CFU,试验组1、5、10、20、50 mg/L浓度时细菌存活率分别为50.3%、22.6%、3.6%、0.3%、0。结论次氯酸钠浓度<50mg/L时对临床常见几种细菌也有较好的敏感性。

Sodium hypochlorite solution on susceptibility of common clinical bacteria

OBJECTIVE To observe the susceptibility of the common bacteria to sodium hypochlorite disinfectant so as to provide theoretical basis for clinical use of chlorine disinfectants.METHODS 0-50 mg/L of six different concentrations of sodium hypochlorite solution was made,a certain amount of Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa,Klebsiella pneumoniae,Acinetobacter baumannii,and Bacillus stearothermophilus were mixed with different concentrations of sodium hypochlorite solution for 5 min,and then were divided into six experimental groups,the inoculation and culture were performed according to conventional method,the effect of sterilization was observed.RESULTS The bacteria of the control group(0 mg / L) developed well,with an average of bacterial colonies of 1123 CFU,the survival rates of bacteria in the study group were respectively 50.3%,22.6%,3.6%,0.3%,and 0 as the concentrations were 1 mg/L,5 mg/L,10 mg/L,20 mg/L,50 mg/L.CONCLUSION When the concentration of sodium hypochlorite is less than 50 mg/L,it has a good susceptibility to several common bacteria.