A-40926混合物中B0组分的分离纯化

目的 开发一种高纯度A-40926混合物中B0组分(以下简称A-40926 B0)的分离纯化工艺。方法 采用中压色谱系统，以聚合物微球为载体，A-40926 B0的收率为指标，筛选聚合物微球的类型，优化洗脱条件。结果 型号为UniPS30-300的微球为最佳层析介质，上样量为10mg(每毫升填料)，洗脱剂依次为体积分数30%，45%的乙醇溶液，流速为1.5BV/h(BV：层析柱体积)；所得产品纯度大于95%，层析总收率大于75%。结论 此种高纯度A-40926 B0的分离纯化方法简单易行，收率稳定，适于工业化生产。

Separation and purification of component B0 in antibiotic A-40926

bjective To develop the separation and purification process of B0 component in the A-40926 mixture (here in after referred to as A-40926 B0). Methods Using medium pressure chromatography system, the polymer microspheres were used as carriers and the yield of A-40926 B0 as indexes. The types of polymer microspheres were screened and the elution conditions were optimized. Results The type of UniPS30-300 polymer particles were selected as the best chromatographic media. The optimal conditions were as follows: The sample loading amount was 10mg (per 1mL filler), and 30% and 45% ethanol solution was used as the elution solution at a flow rate of 1.5BV/h (BV: column volume). The purity of the product was higher than 95% and the yield was above 75%. Conclusion The established purification process of A-40926 B0 was simple to operate and the yield was high and feasible. Thus the process was suitable for industrialized