龟龄集对 AD 小鼠皮层和海马 Fas/ FasL 表达及神经元凋亡的影响

目的 探究龟龄集对阿尔茨海默病 (Alzheimer’s disease, AD) 小鼠皮层和海马 Fas/ FasL 表达及 神经元凋亡的影响。 方法 构建 AD 小鼠模型, 小鼠随机分为对照组、 模型组、 多奈哌齐组及龟龄集低、 中、 高剂量组。 Morris 水迷宫检测干预前后小鼠的学习记忆能力; HE 染色检测小鼠皮层、 海马神经元病理 学改变情况; TUNEL 染色检测神经元凋亡情况; Western 印迹及 Real time PCR 分别检测 Fas、 FasL 的蛋白 表达水平及 mRNA 表达水平。 结果 与模型组比较, 龟龄集各组和多奈哌齐组小鼠学习记忆能力明显提高 (P< 0. 05), 皮层和海马神经元病理学损害及神经元凋亡改善 (P< 0. 05), Fas、 FasL 蛋白和 mRNA 水平下 降 (P< 0. 05)。 结论 龟龄集可能通过抑制 Fas/ FasL 表达抑制 AD 模型小鼠皮层、 海马神经元凋亡, 并改 善其学习记忆能力。

Effect of Guilingji on the Expression of Fas/FasL and Neuronal Apop-tosis in Cortex and Hippocampus of Alzheimer' s Disease Mice

Objective To investigate the effects of Guilingji on the expression of Fas/ FasL and neuronal apoptosis in cortex and hippocampus of mice with Alzheimer’s Disease (AD). Methods AD mouse model was constructed and randomly divided into the model group, donepezil group and low-, medium-and high-dose Guilingji groups. Morris water maze test was used to test the learning and memory ability of mice before and after intervention. HE staining was used to determine the pathological changes of cortical and hippocampal neurons. TUNEL staining was used to measure neuronal apoptosis. Western blotting and real-time PCR were used to detect protein and mRNA expression levels of Fas and FasL. Results The learning and memory ability of mice was significantly improved (P< 0. 05), the pathological damage and apoptosis of neurons in cortex and hippocampus greatly alleviated, and the protein and mRNA expression levels of Fas and FasL markedly decreased in Gulingji groups and donepezil group compared with model group (P < 0. 05 for all). Conclusion Gulingji may inhibit the apoptosis of cortical and hippocampal neurons and improve the learning and memory ability of AD model mice by inhibiting the expression of Fas/ FasL.