留兰香油对慢性阻塞性肺疾病大鼠的气道炎症反应与白细胞介素-8和CCR2受体表达的影响

 目的 观察留兰香油对慢性阻塞性肺疾病(COPD)大鼠气道炎症反应与白细胞介素（IL）-8和CC-亚族超化因子受体2（CCR2）受体表达的影响。方法 用反复被动吸烟和气道内滴入脂多糖12周建立大鼠COPD模型后，留兰香油灌胃治疗4周。测定气道阻力，计数大鼠COPD模型支气管肺泡灌洗液(BALF)中白细胞总数及分类，肺组织病理切片HE、AB-PAS和Masson染色观察气道炎症并行半定量评分及病理图像的形态学测量分析，测定IL-8含量和CCR2的表达水平。结果 留兰香油30、100和300 mg·kg-1能明显降低BALF中白细胞总数、分类中中性粒细胞、淋巴细胞及单核巨噬细胞；减轻细支气管炎和肺间质炎及炎症细胞浸润；能明显减少肺泡破坏，使细支气管壁变薄以及抑制杯状细胞增生，能使气道壁胶原沉积厚度明显降低。留兰香油100和300 mg·kg-1能明显降低肺组织匀浆中的IL-8含量，留兰香油30、100和300 mg·kg-1能明显降低肺组织CCR2表达水平。结论 留兰香油对熏烟和脂多糖引起的大鼠COPD模型的肺部炎症、肺气肿、杯状细胞增生、胶原沉积增厚、气道重塑有改善作用，其机制可能是留兰香油降低了肺组织IL-8的含量和CCR2受体的表达。

Effect of Spearmint Oil on Airway Inflammation,Interleukin-8 and CCR2 Expression in Lung Tissue of COPD Rats

OBJECTIVE To investigate the effect of spearmint oil on airway inflammation and the expression of interleukin-8(IL-8) and CC-chemokines receptors 2(CCR2) in rats with chronic obstructive pulmonary disease(COPD).METHODS COPD model was induced by intratracheal instillation of lipopolysaccharide(LPS) and passive smoking for 12 weeks in rats,and COPD rats were treated with spearmint oil for 4 weeks.After COPD was induced,the methacholine (Mch)-induced airway resistance (RL) and total and differential cell count...