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天津市蓟县地区莱姆病螺旋体主要生物媒介的调查研究
引用本文:侯学霞,刘义,郝琴,陈继永,耿震,宋春英,陈建,丁健青,于凤泰,贾艳合,赵志武,万康林.天津市蓟县地区莱姆病螺旋体主要生物媒介的调查研究[J].中国预防医学杂志,2008,9(5):358-359.
作者姓名:侯学霞  刘义  郝琴  陈继永  耿震  宋春英  陈建  丁健青  于凤泰  贾艳合  赵志武  万康林
作者单位:1. 中国疾病预防控制中心传染病预防控制所,北京,100050
2. 天津市蓟县卫生防病站
3. 中国疾病预防控制中心传染病预防控制所,北京,100050;新乡医学院
4. 天津市疾病预防控制中心
摘    要:目的了解当地莱姆病的主要生物媒介一蜱的种类、带菌状况以及在传播莱姆病中的作用。方法采用布旗法及动物诱捕法在山林地区采集蜱。对蜱种进行鉴定。并进行病原分离培养和PCR检测。结果共收集到1226只蜱,经鉴定属于硬蜱科中2属2种:硬蜱届的全沟硬蜱和血蜱属的长角血蜱。其中以长角血蜱为优势种,构成比占92.58%(1135/1226);随机对300只长角血蜱进行针对莱姆病螺旋体的PCR.检测,有14只阳性,阳性率为4.67%。并对其余926只蜱进行病原分离培养,未得到莱姆病螺旋体。结论血蜱可能是天津蓟县地区莱姆病传播的主要生物媒介。

关 键 词:莱姆病螺旋体  生物媒介  带菌状况  预防措施  传染病

Investigation on Primary Vectors of Borrelia Burgdorferi in Ji County of Tianjin City
Affiliation:HOU Xue - xia , LIU Yi, HAO Qin, et al . ( National Institute of Communicable Disease Control and Prevention, Chinese Ceuter for Disease Control and Prevention, Beijing 100050, China)
Abstract:Objective To explore the species, bacteria carrying status and the role in causing Lyme disease of ticks. Methods ① Flagging and trapping methods were used to collect ticks in forest area and then the species of captured ticks were identified. ② BSK Ⅱ culture medium was used to isolate the pathogen. ③ PCR was used to detect the germ - carrying rate of ticks. Results A total of 1 226 ticks were collected, and then were indentified as Ixodidae (2 genera, 2 species). Haemaphysalis longicornis was the dominant specie. The carrying -rate of Haemaphysalis longicornis in ticks was 4. 67% by PCR, but no pathogen was found by isolation or culture. Conclusion Haemaphysalis longicornis was an important vector in Ji County of Tianjin city.
Keywords:Borrelia burgdorferi  Biological vector
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